A low stringency polymerase chain reaction approach to the identification of Biomphalaria glabrata and B-tenagophila, intermediate snail hosts of Schistosoma mansoni in Brazil

被引：15

作者：

Vidigal, THDA

Neto, ED

Simpson, AJG

Carvalho, OS

机构：

[1] FIOCRUZ MS,CTR PESQUISAS RENE RACHOU,LAB PARASITOL CELULAR & MOL,BR-30190002 BELO HORIZONT,MG,BRAZIL

[2] UNIV FED MINAS GERAIS,ICB,DEPT BIOQUIM & IMUNOL,BELO HORIZONT,MG,BRAZIL

[3] INST LUDWIG PESQUISAS CANC,LAB GENET CANC,SAO PAULO,BRAZIL

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1996年 / 91卷 / 06期

关键词：

polymerase chain reaction; low stringency PCR; Biomphalaria; identification; Brazil;

D O I：

10.1590/S0074-02761996000600016

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The low stringency-polymerase chain reaction (LS-PCR) with a pair of specific primers for the amplification of the 18S rRNA gene was evaluated as a means of differentiating between the two Schistosoma mansoni intermediate host species in Brazil: Biomphalaria glabrata and B. tenagophila. Individual snails obtained from different states of Brazil were used and the amplification patterns obtained showed a high degree of genetic variability in these species. Nevertheless, 4 and 3 clearly defined specific diagnostic bands was observed in individuals from B. glabrata and B. tenagophila respectively. The detection of snail specific diagnostic bands suggests the possibility of reliable species differentiation at the DNA level using LS-PCR.

引用

页码：739 / 744

页数：6

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