Molecular cloning and characterization of a novel angiopoietin family protein, angiopoietin-3

被引：65

作者：

Kim, I

Kwak, HJ

Ahn, JE

So, JN

Liu, MZ

Koh, KN

Koh, GY

机构：

[1] Chonbuk Natl Univ, Sch Med, Natl Creat Res Initiat Ctr Cardiac Regenerat, Chonju 560180, South Korea

[2] Chonbuk Natl Univ, Sch Med, Inst Cardiovasc Res, Chonju 560180, South Korea

[3] Woosuk Univ, Dept Biotechnol, Chonju, South Korea

来源：

FEBS LETTERS | 1999年 / 443卷 / 03期

关键词：

angiopoietin; endothelial cell; vasculogenesis;

D O I：

10.1016/S0014-5793(99)00008-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using homology-based PCR, we have isolated cDNA encoding a novel member (491 amino acids) of the angiopoietin (Ang) family from human adult heart cDNA and have designated it angiopoietin-3 (Ang3). The NH2-terminal and COOH-terminal portions of Ang-3 contain the characteristic coiled-coil domain and fibrinogen-like domain that are conserved in other known Angs. Ang3 has a highly hydrophobic region at the N-terminus (similar to 21 amino acids) that is typical of a signal sequence for protein secretion. Ang3 mRNA is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine in human adult tissues. Additionally, Ang3 is a secretory protein, but is not a mitogen in endothelial cells. (C) 1999 Federation of European Biochemical Societies.

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收藏

页码：353 / 356

页数：4

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