The effect of electroacupuncture on spontaneous recurrent seizure and expression of GAD67 mRNA in dentate gyrus in a rat model of epilepsy

被引：24
作者：
Guo, Jianjun [1 ]
Liu, Jianhua [2 ]
Fu, Wenbin [2 ]
Ma, Wentao [3 ]
Xu, Zhenhua [2 ]
Yuan, Minqquan [1 ]
Song, Jian [4 ]
Hu, Jiming [1 ]
机构：
[1] Wuhan Univ, Coll Chem & Mol Sci, Dept Anal Measurement Sci, Wuhan 430072, Peoples R China
[2] Guangzhou Univ Chinese Med, Sch Clin Med 2, Dept Acupuncture, Guangzhou, Peoples R China
[3] Wuhan Univ, Coll Life Sci, Wuhan, Peoples R China
[4] Wuhan Univ, Coll Basic Med, Wuhan, Peoples R China
来源：
BRAIN RESEARCH | 2008年 / 1188卷
基金：
中国国家自然科学基金;
关键词：
epilepsy; electroacupuncture; acupoint glutamic acid decarboxylase; spontaneous recurrent seizure; dentate gyrus;
D O I：
10.1016/j.brainres.2007.10.017
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Concerns regarding the side effects of pharmacological approaches have recently increased interest in the use of acupuncture for treatment of epilepsy. Although clinical evidence for the acupunctural anti-epileptic effect has been demonstrated, the precise mechanism still remains unknown. The purpose of this study was to investigate the effect of electroacupuncture (EA) on spontaneous recurrent seizure (SRS) and expression of GAD(67) mRNA in dentate gyrus (DG) in epileptic rats. EA at bilateral acupoints of Zusanli (St36) was administered. Two sham EA controls were set: sham EA at bilateral nearby nonacupoints in the hamstring muscles, and sham EA at bilateral St36 without electrical stimulation. Lithium-pilocarpine injection was performed to establish the rat model of epilepsy at the 1st day. Three time points were set according to the day when the rats were killed (30th, 45th, 60th day). The results showed that EA at St36 significantly reduced the times of spontaneous recurrent seizure, neither of the two sham EA controls displayed significant effect on spontaneous recurrent seizure. Moreover, EA at St36 significantly elevated the expression of GAD(67) mRNA in DG granule cell layer (GCL), but not in the hilus; neither of the two sham controls showed significant effect on the expression of GAD(67) mRNA in granule cell layer or hilus. The findings suggest that EA at St36 possess some curative effect on epileptic rats, related with change of GAD(67) mRNA level in DG region. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：165 / 172
页数：8
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