Mass spectrometry-based phytochemical screening for hypoglycemic activity of Fagioli di Sarconi beans (Phaseolus vulgaris L.)

被引：39

作者：

Pascale, Raffaella ^{[1
]}

Bianco, Giuliana ^{[2
]}

Cataldi, Tommaso R. I. ^{[3
]}

Kopplin, Philippe-Schmitt ^{[4
,5
]}

Bosco, Federica ^{[2
]}

Vignola, Lisiana ^{[2
]}

Uhl, Jenny ^{[4
]}

Lucio, Marianna ^{[4
]}

Milella, Luigi ^{[2
]}

机构：

[1] Univ Basilicata, Scuola Ingn, Via Ateneo Lucano 10, I-85100 Potenza, Italy

[2] Univ Basilicata, Dipartimento Sci, Via Ateneo Lucano 10, I-85100 Potenza, Italy

[3] Univ Bari Aldo Moro, Dipartimento Chim, Via E Orabona 4, I-70126 Bari, Italy

[4] Helmholtz Zentrum Muenchen, Analyt BioGeoChem, Ingolstaedter Landstr 1, D-85764 Neuherberg, Germany

[5] Tech Univ Muenchen, Chair Analyt Food Chem, Alte Akad 10, D-85354 Weihenstephan Freising, Germany

来源：

FOOD CHEMISTRY | 2018年 / 242卷

关键词：

Fagioli di Sarconi beans; Phaseouls vulgaris L; High-resolution mass spectrometry; Phytochemical profile; Antidiabetic activity; alpha-Glucosidase; alpha-Amylase; ALPHA-GLUCOSIDASE; ORGANIC-MATTER; ANTIOXIDANT; INHIBITORS; PATHWAYS; SAPONINS; ACARBOSE; SPECTRA; LEGUMES; LTQ;

D O I：

10.1016/j.foodchem.2017.09.091

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The present study deals with the evaluation of antidiabetic activities of Fagioli di Sarconi beans (Phaseolus vulgaris), including 21 ecotypes protected by the European Union with the mark PGI (i.e., Protected Geographical Indication), and cultivated in Basilicata (southern Italy). For this purpose, alpha-glucosidase and alpha-amylase assays were assessed; among all bean ecotypes, the tight green seed colour of Verdolino extracts exhibited the highest alpha-glucosidase and alpha-amylase inhibitory activity with IC50 = 1.1 +/- 0.1 mu g/ml and IC50 = 19.3 +/- 1.1 mu g/ml, respectively. Phytochemical compound screening of all Fagioli di Sarconi beans performed by flow injection-electrospray ionization-ultrahigh resolution mass spectrometry (uHRMS) and based on the calculation of elemental formulas from accurate m/z values, was helpful to annotate specific compounds, such as alkaloids, saponins, flavonoids, and terpenoids, which are most likely responsible for their biological activity.

引用

页码：497 / 504

页数：8

共 40 条

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