Characterization of Three Lactic Acid Bacteria and Their Isogenic ldh Deletion Mutants Shows Optimization for YATP (Cell Mass Produced per Mole of ATP) at Their Physiological pHs

被引：25

作者：

Fiedler, Tomas ^{[1
]}

Bekker, Martijn ^{[2
]}

Jonsson, Maria ^{[3
]}

Mehmeti, Ibrahim ^{[3
]}

Pritzschke, Anja ^{[1
]}

Siemens, Nikolai ^{[1
]}

Nes, Ingolf ^{[3
]}

Hugenholtz, Jeroen ^{[2
]}

Kreikemeyer, Bernd ^{[1
]}

机构：

[1] Univ Rostock, Inst Med Microbiol Virol & Hyg, D-18057 Rostock, Germany

[2] Univ Amsterdam, Swammerdam Inst Life Sci, Dept Microbiol, NL-1018 WV Amsterdam, Netherlands

[3] Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, N-1432 As, Norway

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

LACTOCOCCUS-LACTIS; ENTEROCOCCUS-FAECALIS; STREPTOCOCCUS-PYOGENES; LACTATE-DEHYDROGENASE; ESCHERICHIA-COLI; CHEMOSTAT CULTURES; GENOME SEQUENCE; GLUCOSE; GROWTH; FERMENTATION;

D O I：

10.1128/AEM.01838-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several lactic acid bacteria use homolactic acid fermentation for generation of ATP. Here we studied the role of the lactate dehydrogenase enzyme on the general physiology of the three homolactic acid bacteria Lactococcus lactis, Enterococcus faecalis, and Streptococcus pyogenes. Of note, deletion of the ldh genes hardly affected the growth rate in chemically defined medium under microaerophilic conditions. However, the growth rate was affected in rich medium. Furthermore, deletion of ldh affected the ability for utilization of various substrates as a carbon source. A switch to mixed acid fermentation was observed during glucose-limited continuous growth and was dependent on the growth rate for S. pyogenes and on the pH for E. faecalis. In S. pyogenes and L. lactis, a change in pH resulted in a clear change in Y(ATP) (cell mass produced per mole of ATP). The pH that showed the highest Y(ATP) corresponded to the pH of the natural habitat of the organisms.

引用

页码：612 / 617

页数：6

共 30 条

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