Grating light reflection spectroscopy for determination of bulk refractive index and absorbance

被引:16
作者
Anderson, BB [1 ]
Brodsky, AM [1 ]
Burgess, LW [1 ]
机构
[1] UNIV WASHINGTON, DEPT CHEM, SEATTLE, WA 98195 USA
关键词
D O I
10.1021/ac951177s
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An optical sensing technique is described and evaluated for sensitivity to changes in refractive index and absorbance of model sample matrices, A binary dielectric/metal transmission diffraction grating is placed in contact with a sample and utilized in reflection mode; thus, the light captured and analyzed does not pass through the sample, This particular condition creates thresholds at which a particular transmitted diffraction order is transformed from a traveling wave to an evanescent one, The positions of these thresholds depend upon the complex dielectric function of the sample, the period of the grating, and the wavelength and incident angle of light striking the grating, Experimental evidence directly supports the theoretical predictions regarding responses to both the real and imaginary portions of the refractive index: the reflection coefficient derivative wavelength peak position shifts linearly with changes in the real part of the refractive index, and the derivative peak amplitudes exhibit a square-root dependence on absorbance, Refractive index sensitivity to a series of ethanol/water solutions is demonstrated with detectable changes in index as small as 2 x 10(-6). Absorbance sensitivity is shown via the differentiation of methylene blue samples having equivalent 1 cm path length absorbances between 0.459 and 244 AU. In a single reflection measurement, GLRS offers a large dynamic range for absorbance detection, allows simultaneous determination of bulk refractive index in optically dense media, and provides a platform for performing continuous process analysis.
引用
收藏
页码:1081 / 1088
页数:8
相关论文
共 17 条
[1]  
ANDERSON BB, 1995, UNPUB PHYS REV
[2]  
ANDERSON BB, IN PRESS J PROCESS A
[3]  
CONN HJ, 1953, BIOL STAINS, P111
[4]   DIFFRACTION EFFICIENCY OF BINARY METAL GRATINGS WORKING IN TOTAL INTERNAL-REFLECTION [J].
DUSHKINA, N ;
SAINOV, S .
JOURNAL OF MODERN OPTICS, 1992, 39 (01) :173-187
[5]   DEVELOPMENT OF A PROTOTYPE GAS SENSOR USING SURFACE-PLASMON RESONANCE ON GRATINGS [J].
JORY, MJ ;
VUKUSIC, PS ;
SAMBLES, JR .
SENSORS AND ACTUATORS B-CHEMICAL, 1994, 17 (03) :203-209
[6]   CHEMOMETRIC EVALUATION OF THE MULTIPLE-MODE RESPONSE OF AN ION-DIFFUSED PLANAR OPTICAL WAVE-GUIDE TO LIQUID-PHASE ANALYTES [J].
KUHN, KJ ;
BURGESS, LW .
ANALYTICAL CHEMISTRY, 1993, 65 (10) :1390-1398
[7]   SENSITIVITY OF INTEGRATED OPTICAL GRATING AND PRISM COUPLERS AS (BIO)CHEMICAL SENSORS [J].
LUKOSZ, W ;
TIEFENTHALER, K .
SENSORS AND ACTUATORS, 1988, 15 (03) :273-284
[8]  
Lukosz W., 1983, IEE C PUBL LONDON, V227, P152
[9]  
NEWTON RG, 1966, SCATTERING THEORY WA, pCH17
[10]  
RYTOV SM, 1950, ZH EKSP TEOR FIZ, V20, P340