Archaeal ADP-dependent phosphofructokinase: expression, purification, crystallization and preliminary crystallographic analysis

被引：3

作者：

Jeong, JJ ^{[1
]}

Fushinobu, S ^{[1
]}

Ito, S ^{[1
]}

Hirofumi, S ^{[1
]}

Wakagi, T ^{[1
]}

机构：

[1] Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903010631

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Thermococcus litoralis uses a modified Embden-Meyerhof pathway Its phosphofructokinase (TLPFK) catalyses the phosphorylation of fructose-6-phosphate by ADP, but not by ATP, in the presence of Mg2+, yielding fructose-1,6-bisphosphate. The gene encoding TLPFK was cloned and overexpressed in Escherichia coli. Recombinant TLPFK consists of a dimer with a 52 kDa subunit. The native crystals belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 85.41, c = 163.93 Angstrom, and diffract to beyond 2.6 Angstrom resolution. The TLPFK structure was preliminarily analyzed by means of multiple isomorphous replacement with four heavy-atom derivatives.

引用

页码：1327 / 1329

页数：3

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