Comparison of fluorescence in situ hybridisation using peptide nucleic acid probes, Gram stain/acridine orange leukocyte cytospin and differential time to positivity methods for detection of catheter-related bloodstream infection in patients after haematopoietic stem cell transplantation

被引：0

作者：

Krause, R. ^{[1
]}

Salzer, H. F. ^{[1
]}

Hoenigl, M. ^{[1
]}

Valentin, T. ^{[1
]}

Auner, H. W. ^{[2
]}

Zollner-Schwetz, I. ^{[1
]}

机构：

[1] Med Univ Graz, Dept Internal Med, Infect Dis Sect, Div Pulmonol, A-8036 Graz, Austria

[2] Univ London Imperial Coll Sci Technol & Med, Med Res Council Clin Sci Ctr, London, England

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2010年 / 16卷 / 10期

关键词：

RAPID DIAGNOSIS; CULTURES; STAIN; DISEASES;

D O I：

10.1111/j.1469-0691.2010.03154.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In 46 febrile neutropenic patients who had undergone haematopoietic stem cell transplantation, the fluorescence in situ hybridisation using peptide nucleic acid probes (PNA FISH), Gram stain/acridine orange leukocyte cytospin (Gram/AOLC), and differential time to positivity (DTP) methods were performed for detection of catheter-related bloodstream infections (CRBSIs). As compared with the DTP method (which detected 11 patients with CRBSI), the PNA FISH and the Gram/AOLC methods detected ten of 11 CRBSI patients, resulting in a sensitivity, specificity, negative predictive value and positive predictive value of 91%, 100%, 97% and 100%, respectively, for the PNA FISH method as well as for the Gram/AOLC method.

引用

页码：1591 / 1593

页数：3