Overproduction and purification of the CcpA protein from Lactococcus lactis

被引：0
|
作者：
Kowalczyk, M [1 ]
Bardowski, J [1 ]
机构：
[1] PAS, Inst Biochem & Biophys, Dept Microbial Biochem, PL-02106 Warsaw, Poland
来源：
ACTA BIOCHIMICA POLONICA | 2003年 / 50卷 / 02期
关键词：
CcpA; His-tag; cross links; dimers;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6 x His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 mug/ml ampicillin at 37degreesC and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.
引用
收藏
页码：455 / 459
页数：5
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