Study on the interaction between nucleic acid and Eu3+-oxolinic acid and the determination of nucleic acid using the resonance light scattering technique

被引：11
作者：
Wu, X
Sun, SN
Yang, JH [1 ]
Wang, MQ
Liu, LY
Guo, CY
机构：
[1] Shandong Univ, Sch Chem & Chem Engn, Key Lab Colloid Interface Chem, Minist Educ, Jinan 250100, Peoples R China
[2] Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China
[3] Jinan Petrochem Design Inst, Jinan 250100, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2005年 / 62卷 / 4-5期
关键词：
nucleic acids; oxolinic acid; resonance light scattering;
D O I：
10.1016/j.saa.2005.03.022
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
At pH 9.75, the resonance light scattering (RLS) intensity of OA-Eu3+ system is greatly enhanced by nucleic acid. Based on this phenomenon, a new quantitative method for nucleic acid in aqueous solution has been developed. Under the optimum condition, the enhanced RLS is proportional to the concentration of nucleic acid in the range of 1.0 x 10(-9) to 1.0 x 10(-6) g/ml for herring sperm DNA, 8.0 x 10(-10) to 1.0 x 10(-6) g/ml for calf thymus DNA and 1.0 x 10(-9) to 1.0 x 10(-6) g/ml for yeast RNA, and their detection limits are 0.020, 0.011 and 0.010 ng/ml, respectively. Synthetic samples and actual samples were satisfactorily determined. In addition, the interaction mechanism between nucleic acid and OA-Eu3+ is also investigated. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：896 / 901
页数：6
相关论文
共 26 条
[1]  
BAO A, 2002, ANAL CHEM, V74, P172
[2]  
Chen XL, 2001, CHEM J CHINESE U, V22, P901
[3]   Resonance light scattering technique for the determination of proteins with resorcinol yellow and OP [J].
Chen, YJ ;
Yang, JH ;
Wu, X ;
Wu, T ;
Luan, YX .
TALANTA, 2002, 58 (05) :869-874
[4]   FLUORESCENCE REACTION OF TERBIUM(III) WITH NUCLEIC-ACIDS IN THE PRESENCE OF PHENANTHROLINE [J].
CI, YX ;
LI, YZ ;
CHANG, WB .
ANALYTICA CHIMICA ACTA, 1991, 248 (02) :589-594
[5]   Sensitive and simple determination of protein by resonance Rayleigh scattering with 4-azochromotropic acid phenylfluorone [J].
Guo, ZX ;
Shen, HX .
ANALYTICA CHIMICA ACTA, 2000, 408 (1-2) :177-182
[6]   Determination of nanograms of nucleic acids by their enhancement effect on the resonance light scattering of the cobalt(II)/4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene complex [J].
Huang, CZ ;
Li, KA ;
Tong, SY .
ANALYTICAL CHEMISTRY, 1997, 69 (03) :514-520
[7]   Determination of nucleic acids by a resonance light-scattering technique with alpha,beta,gamma,delta-tetrakis[4-(trimothylammoniumyl)phenyl]porphine [J].
Huang, CZ ;
Li, KA ;
Tong, SY .
ANALYTICAL CHEMISTRY, 1996, 68 (13) :2259-2263
[8]   SIMPLE, RAPID, AND SENSITIVE DNA ASSAY PROCEDURE [J].
LABARCA, C ;
PAIGEN, K .
ANALYTICAL BIOCHEMISTRY, 1980, 102 (02) :344-352
[9]   A NEW FLUOROMETRIC METHOD FOR RNA AND DNA DETERMINATION [J].
LEPECQ, JB ;
PAOLETTI, C .
ANALYTICAL BIOCHEMISTRY, 1966, 17 (01) :100-+
[10]   A novel fluorometric method for DNA and RNA determination [J].
Li, WY ;
Xu, JG ;
Guo, XQ ;
Zhu, QZ ;
Zhao, YB .
ANALYTICAL LETTERS, 1997, 30 (03) :527-536
123