Integration of glass micropipettes with a 3D printed aligner for microfluidic flow cytometer

被引：21

作者：

Bayram, Abdullah ^{[1
]}

Serhatlioglu, Murat ^{[2
]}

Ortac, Bulend ^{[2
]}

Demic, Serafettin ^{[1
]}

Elbuken, Caglar ^{[2
]}

Sen, Mustafa ^{[3
]}

Solmaz, Mehmet Ertugrul ^{[4
]}

机构：

[1] Izmir Katip Celebi Univ, Dept Mat Sci & Engn, Izmir, Turkey

[2] Bilkent Univ, Inst Mat Sci & Nanotechnol, Natl Nanotechnol Res Ctr, UNAM, TR-06800 Ankara, Turkey

[3] Izmir Katip Celebi Univ, Dept Biomed Engn, Izmir, Turkey

[4] Izmir Katip Celebi Univ, Dept Elect & Elect Engn, Izmir, Turkey

来源：

SENSORS AND ACTUATORS A-PHYSICAL | 2018年 / 269卷

关键词：

Flow cytometry; Hydrodynamic focusing; Micropipette; 3D printing; Optofluidics; CELL ANALYSIS; DEVICE; SENSITIVITY; DIAGNOSIS;

D O I：

10.1016/j.sna.2017.11.056

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

In this study, a facile strategy for fabricating a microfluidic flow cytometer using two glass micropipettes with different sizes and a 3D printed millifluidic aligner was presented. Particle confinement was achieved by hydrodynamic focusing using a single sample and sheath flow. Device performance was extracted using the forward and side-scattered optical signals obtained using fiber-coupled laser and photodetectors. The 3-D printing assisted glass capillary microfluidic device is ultra-low-cost, not labor-intensive and takes less than 10 min to fabricate. The present device offers a great alternative to conventional benchtop flow cytometers in terms of optofluidic configuration. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：382 / 387

页数：6

共 39 条

[1]

[Anonymous], J MICROMECH MICROENG

[2] Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping [J].

Augustsson, Per ;

Karlsen, Jonas T. ;

Su, Hao-Wei ;

Bruus, Henrik ;

Voldman, Joel .

NATURE COMMUNICATIONS, 2016, 7

[3] Discrete elements for 3D microfluidics [J].

Bhargava, Krisna C. ;

Thompson, Bryant ;

Malmstadt, Noah .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (42) :15013-15018

[4] The upcoming 3D-printing revolution in microfluidics [J].

Bhattacharjee, Nirveek ;

Urrios, Arturo ;

Kanga, Shawn ;

Folch, Albert .

LAB ON A CHIP, 2016, 16 (10) :1720-1742

[5] Standing surface acoustic wave (SSAW)-based microfluidic cytometer [J].

Chen, Yuchao ;

Nawaz, Ahmad Ahsan ;

Zhao, Yanhui ;

Huang, Po-Hsun ;

McCoy, J. Phillip ;

Levine, Stewart J. ;

Wang, Lin ;

Huang, Tony Jun .

LAB ON A CHIP, 2014, 14 (05) :916-923

[6] A three-dimensional (3D) particle focusing channel using the positive dielectrophoresis (pDEP) guided by a dielectric structure between two planar electrodes [J].

Chu, Hyunjung ;

Doh, Il ;

Cho, Young-Ho .

LAB ON A CHIP, 2009, 9 (05) :686-691

[7] Diagnostic Flow Cytometry and the AIDS Pandemic [J].

Clift, Ian C. .

LABMEDICINE, 2015, 46 (03) :E59-E64

[8] Lymph Node Fine-Needle Cytology of Non-Hodgkin Lymphoma: Diagnosis and Classification by Flow Cytometry [J].

Cozzolino, Immacolata ;

Rocco, Monia ;

Villani, Giancarlo ;

Picardi, Marco .

ACTA CYTOLOGICA, 2016, 60 (04) :302-314

[9] Single-layer microfluidic device to realize hydrodynamic 3D flow focusing [J].

Eluru, Gangadhar ;

Julius, Lourdes Albina Nirupa ;

Gorthi, Sai Siva .

LAB ON A CHIP, 2016, 16 (21) :4133-4141

[10] Flow Cytometry Can Diagnose Classical Hodgkin Lymphoma in Lymph Nodes With High Sensitivity and Specificity [J].

Fromm, Jonathan R. ;

Thomas, Anju ;

Wood, Brent L. .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2009, 131 (03) :322-332

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