Synthesis of porous macrospheres from amino-polymers

被引：10

作者：

Baltieri, RC

Innocentini-Mei, LH

Tamashiro, WMSC

Peres, L

Bittencourt, E

机构：

[1] Univ Estadual Campinas, UNICAMP, Fac Engn Quim, Dept Tecnol Polimeros, BR-13081970 Campinas, SP, Brazil

[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Imunol, BR-13081970 Campinas, SP, Brazil

来源：

EUROPEAN POLYMER JOURNAL | 2002年 / 38卷 / 01期

关键词：

amino-polymers; macrospheres; immunoassays;

D O I：

10.1016/S0014-3057(01)00177-X

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Porous macrospheres with controlled diameter were produced using N-methylolacrylamide and urea-formaldehyde resins synthesized in our laboratories. Hot silicon oil was used to form the porous macrospheres which were characterized by using infrared spectroscopy (FTIR), scanning electron microscopy, mercury porosimetry and surface area determination (BET). Porous macrospheres of amino-polymers were used as support in immunoassays type ELISA (enzyme linked immunosorbent assay) in which mouse immunoglobulin (IgG) was immobilized, and detected by rabbit anti-mouse immunoglobulin (a-IgG) labeled with horseradish peroxidase. Responses were obtained up to a dilution ratio of 1:4000 of the conjugate standard (1 mg/ml) and 10 mug/ml of IgG solution to recover the spheres in pH 4.5. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：57 / 62

页数：6

共 18 条

[1] Immobilization of pectin lyase from Penicillium italicum by covalent binding to nylon [J].

Alkorta, I ;

Garbisu, C ;

Llama, MJ ;

Serra, JL .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (02) :141-146

[2] Covalent immobilization of acid phosphatase on amorphous AlPO4 support [J].

Bautista, FM ;

Bravo, MC ;

Campelo, JM ;

Garcia, A ;

Luna, D ;

Marinas, JM ;

Romero, AA .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1999, 6 (05) :473-481

[3]

Bulmus V, 1997, CHEM ENG J, V65, P71, DOI 10.1016/S0923-0467(96)03156-9

[4]

CABRAL JMS, 1982, THESIS U TECHNICA LI

[5]

CABRAL JMS, 1982, THESIS U TECNICA LIS

[6]

CARDOSO VL, 1988, THESIS UNICAMP SP BR

[7]

CARDOSO VL, 1988, SINTESE ESTUDO DESEM

[8]

Chang T. M. S., 1977, BIOMEDICAL APPL IMMO, V2

[9]

EBDON JR, 1991, NEW METHODS POLYM SY

[10]

Eldin MSM, 2000, J MOL CATAL B-ENZYM, V10, P445

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