Investigation by fluorescence correlation spectroscopy of the chaperoning interactions of HIV-1 nucleocapsid protein with the viral DNA initiation sequences

被引：18

作者：

Égelé, C ^{[1
]}

Schaub, E ^{[1
]}

Piémont, É ^{[1
]}

de Rocquigny, H ^{[1
]}

Mély, Y ^{[1
]}

机构：

[1] Univ Louis Pasteur Strasbourg 1, Lab Pharmacol & Phys Chim Interact Cellulaires &, UMR 7034, CNRS,Fac Pharm, F-67401 Illkirch Graffenstaden, France

来源：

COMPTES RENDUS BIOLOGIES | 2005年 / 328卷 / 12期

关键词：

kissing complexes; stem-loop; fraying; time-resolved fluorescence; fluorescence correlation spectroscopy;

D O I：

10.1016/j.crvi.2005.06.005

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

HIV-1 nucleocapsid protein (NC) exhibits nucleic acid chaperone properties that are important during reverse transcription. Herein, we review and extend our recent investigation by fluorescence correlation spectroscopy (FCS) of the NC chaperone activity on the primer binding site sequences (PBS) of the (-) and (+) DNA strands, which are involved in the second strand transfer during reverse transcription. In the absence of NC, the PBS stem-loops exhibited a fraying limited to the terminal G-C base pair. The kinetics of fraying were significantly activated by NC, a feature that may favour (-)PBS/(+)PBS annealing during the second strand transfer. In addition, NC was found to promote the formation of PBS kissing homodimers through interaction between the loops. These kissing complexes may favour secondary contacts between viral sequences and thus, promote recombination and viral diversity.

引用

页码：1041 / 1051

页数：11

共 57 条

[1] Role of post-transcriptional modifications of primer tRNALys,3 in the fidelity and efficacy of plus strand DNA transfer during HIV-1 reverse transcription [J].