Adenosine deaminase activity in the serum and malignant tumors of breast cancer: The assessment of isoenzyme ADA1 and ADA2 activities

被引：50
作者：
Aghaei, M
Karami-Tehrani, F
Salami, S
Atri, M
机构：
[1] Tarbiat Modares Univ, Sch Med Sci, Dept Clin Biochem, Tehran, Iran
[2] Univ Tehran Med Sci, Dept Surg, Sch Med Sci, Tehran, Iran
来源：
CLINICAL BIOCHEMISTRY | 2005年 / 38卷 / 10期
关键词：
adenosine deaminase; isoenzyme; breast cancer;
D O I：
10.1016/j.clinbiochem.2005.05.015
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objective: The potential relationship between adenosine deaminase activity and cancer progression was examined by investigating the activity of total ADA and its isoenzymes in serum and simultaneously in the cancerous tissue of each patient with breast cancer. Methods: Total ADA and its isoenzymes were measured using the Giusti method. ADA2 activity was measured in the presence of a specific ADA1 inhibitor, EHNA. Results: Our results indicated that ADA2 and total ADA activities were higher in serum and malignant tissues than those of corresponding controls (P < 0.05). Tumor ADA2 and total ADA activities were significantly (P < 0.05) correlated with lymph node involvement, histological grade and tumor size, whereas their levels in serum were significantly (P < 0.05) correlated with menopausal status and patient age. Conclusion: Although serum and tumor total ADA activity and its ADA2 isoenzyme were both found to be increased, distinct correlation patterns were observed with some of the prognostic factors. It can be speculated that increased ADA and isoenzyme activities in serum originated from sources other than the breast tumors. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved.
引用
收藏
页码：887 / 891
页数：5
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