Isolation and characterization of polysaccharides of a hybrid mushroom (backcross mating between PfloVv12 and Volvariella volvacea)

被引：23

作者：

Nandan, Chanchal K. ^{[1
]}

Sarkar, Ramsankar ^{[1
]}

Bhanja, Sunil K. ^{[1
]}

Sikdar, Samir R. ^{[2
]}

Islam, Syed S. ^{[1
]}

机构：

[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India

[2] Bose Inst, Plant Mol & Cellular Genet Sect, Kolkata 700054, W Bengal, India

来源：

CARBOHYDRATE RESEARCH | 2011年 / 346卷 / 15期

关键词：

Hybrid mushroom; Polysaccharide; Structure; NMR spectroscopy; PLEUROTUS-FLORIDA; EDIBLE MUSHROOM; STRUCTURAL-CHARACTERIZATION; AQUEOUS EXTRACT; ANTITUMOR; BODIES; FUNGI;

D O I：

10.1016/j.carres.2011.08.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three polysaccharide fractions (PS-I. PS-II, and PS-Ill) were isolated from the aqueous extract of a hybrid mushroom obtained through backcross mating of a somatic hybrid mushroom PfloVv12 (Sterile line) with Volvariella volvacea. PfloVv12 was obtained through protoplast fusion of Pleurotus florida and V. volvacea. PS-I was identified as 1,6-beta glucan. PS-II and PS-Ill were identified as mannoglucogalactan but differing in molecular weights only. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiment (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structures of these polysaccharides were established as; [GRAPHICS] (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：2451 / 2456

页数：6

共 26 条

[1] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].

AGRAWAL, PK .

PHYTOCHEMISTRY, 1992, 31 (10) :3307-3330

[2] Structural characterization of an immunoenhancing heteroglycan isolated from an aqueous extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer [J].

Bhunia, Sanjoy Kumar ;

Dey, Biswajit ;

Maity, Kankan K. ;

Patra, Sukesh ;

Mandal, Soumitra ;

Maiti, Swatilekha ;

Maiti, Tapas K. ;

Sikdar, Samir R. ;

Islam, Syed S. .

CARBOHYDRATE RESEARCH, 2010, 345 (17) :2542-2549

[3] Production and characterization of somatic hybrids raised through protoplast fusion between edible mushroom strains Volvariella volvacea and Pleurotus florida [J].

Chakraborty, Uttara ;

Sikdar, Samir Ranjan .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2008, 24 (08) :1481-1492

[4] A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].

CIUCANU, I ;

KEREK, F .

CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217

[5] A (1→6)-β-glucan from a somatic hybrid of Pleurotus florida and Volvariella volvacea: isolation, characterization, and study of immunoenhancing properties [J].

Das, Debsankar ;

Mondal, Subhas ;

Roy, Sadhan K. ;

Maiti, Debabrata ;

Bhunia, Bibhas ;

Maiti, Tapas K. ;

Sikdar, Samir R. ;

Islam, Syed S. .

CARBOHYDRATE RESEARCH, 2010, 345 (07) :974-978

[6]

Etsu K., 1989, CARBOHYD RES, V193, P227

[7] POLYSACCHARIDES IN PHARMACY - CURRENT APPLICATIONS AND FUTURE CONCEPTS [J].

FRANZ, G .

PLANTA MEDICA, 1989, (06) :493-497

[8] DETERMINATION OF D AND L CONFIGURATION OF NEUTRAL MONOSACCHARIDES BY HIGH-RESOLUTION CAPILLARY GLC [J].

GERWIG, GJ ;

KAMERLING, JP ;

VLIEGENTHART, JFG .

CARBOHYDRATE RESEARCH, 1978, 62 (02) :349-357

[9]

GOLDSTEIN I. J., 1965, METHOD CARBOHYD CHEM, V5, P361

[10] POLYSACCHARIDES IN FUNGI .9. ANTI-INFLAMMATORY ACTIVITY AND CONFORMATIONAL BEHAVIOR OF A BRANCHED (1-]3)-BETA-D-GLUCAN FROM AN ALKALINE EXTRACT OF DICTYOPHORA-INDUSIATA FISCH [J].

HARA, C ;

KIHO, T ;

TANAKA, Y ;

UKAI, S .

CARBOHYDRATE RESEARCH, 1982, 110 (01) :77-87

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