Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain

被引：135
作者：
Barbosa, Joana S. [1 ,2 ]
Sanchez-Gonzalez, Rosario [1 ]
Di Giaimo, Rossella [1 ,3 ]
Baumgart, Emily Violette [1 ]
Theis, Fabian J. [4 ,5 ]
Goetz, Magdalena [1 ,6 ,7 ]
Ninkovic, Jovica [1 ,6 ]
机构：
[1] Helmholtz Ctr Munich, Inst Stem Cell Res, Munich, Germany
[2] Univ Coimbra, Ctr Neurosci & Cell Biol, PhD Program Biomed & Expt Biol BEB, Coimbra, Portugal
[3] Univ Naples Federico II, Dept Biol, Naples, Italy
[4] Helmholtz Ctr Munich, Inst Computat Biol, Munich, Germany
[5] Tech Univ Munich, Inst Math Sci, Garching, Germany
[6] Univ Munich, Biomed Ctr, Munich, Germany
[7] Univ Munich, Munich Cluster Syst Neurol SyNergy, Munich, Germany
来源：
SCIENCE | 2015年 / 348卷 / 6236期
基金：
欧洲研究理事会; 芬兰科学院;
关键词：
TELENCEPHALON; REGENERATION; NEUROGENESIS; DIFFERENTIATION; QUIESCENCE; FOREBRAIN; MODEL; ZONE;
D O I：
10.1126/science.aaa2729
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Adult neural stem cells are the source for restoring injured brain tissue. We used repetitive imaging to follow single stem cells in the intact and injured adult zebrafish telencephalon in vivo and found that neurons are generated by both direct conversions of stem cells into postmitotic neurons and via intermediate progenitors amplifying the neuronal output. We observed an imbalance of direct conversion consuming the stem cells and asymmetric and symmetric self-renewing divisions, leading to depletion of stem cells over time. After brain injury, neuronal progenitors are recruited to the injury site. These progenitors are generated by symmetric divisions that deplete the pool of stem cells, a mode of neurogenesis absent in the intact telencephalon. Our analysis revealed changes in the behavior of stem cells underlying generation of additional neurons during regeneration.
引用
收藏
页码：789 / 793
页数：5
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