Simultaneous Determination of Glucuronic Acid, Malic Acid, Taurine and Potassium Sorbate in Functional Drinks by Ion Chromatography

被引:0
作者
Sha Ruyi [1 ,2 ,3 ]
Cai Chenggang [1 ,2 ,3 ]
机构
[1] Zhejiang Univ Sci & Technol, Sch Light Ind, Sch Biol & Chem Engn, Hangzhou 310023, Zhejiang, Peoples R China
[2] Zhejiang Prov Key Lab Chem & Biol Proc Technol Fa, Hangzhou 310023, Zhejiang, Peoples R China
[3] Zhejiang Collaborat Innovat Ctr Chem & Biol Mfg A, Hangzhou 310023, Zhejiang, Peoples R China
来源
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2016年 / 11卷 / 10期
关键词
Functional drinks; glucuronic acid; ion chromatography; malic acid; potassium sorbate and taurine; PERFORMANCE LIQUID-CHROMATOGRAPHY; ENERGY DRINKS; CAFFEINE; HPLC; FOOD;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A method for simultaneous determination of four acidic ingredients of glucuronic acid, malic acid, taurine and potassium sorbate in functional drinks was developed by ion chromatography. The mobile phase of 20 mmol.L-1 KOH in 1.0 mL.min(-1) was run for 20 min through the anion separation column at 35 degrees C. The anion suppressor current and the cell temperature were set at 50 mA and 30 degrees C respectively. This method is able to separate and analyze four components of glucuronic acid, malic acid, taurine and potassium sorbate at the same time with their limits of detection of 0.72, 0.64, 2.58 and 1.5 mu g.mL(-1) and the corresponding correlation coefficient of 0.999, 0.996, 0.989 and 0.958 respectively. The average recovery range was of 94-104% with the relative standard deviations of 0.77-1.08% (n=6). The established ion chromatography method can be used in the acid drinks analysis and quality control during the processing and storage of functional drinks.
引用
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页码:22 / 26
页数:5
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