Effect of conceptus secretions on HOXA10 and PTGS2 gene expression, and PGE2 release in co-cultured luminal epithelial and stromal cells of the porcine endometrium at the time of early implantation

被引:29
作者
Blitek, A. [1 ]
Morawska, E. [1 ]
Kiewisz, J. [1 ]
Ziecik, A. J. [1 ]
机构
[1] Polish Acad Sci, Inst Anim Reprod & Food Res, PL-10747 Olsztyn, Poland
关键词
Pig; Endometrium; Pregnancy; Conceptus; HOXA10; Steroids; Prostaglandins; EARLY-PREGNANCY; ESTROUS-CYCLE; DIETHYLSTILBESTROL DES; PIG ENDOMETRIUM; IN-VITRO; PROGESTERONE; PROSTAGLANDIN-F2-ALPHA; BLASTOCYST; MICE; STEROIDS;
D O I
10.1016/j.theriogenology.2011.05.002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Homeobox A10 (HOXA10) gene expression was demonstrated in the endometrium of adult porcine uteri, however there is little information concerning the role of this gene in the pig. Objectives of the present study were to examine: 1) the expression of HOXA10 in the endometrium of cyclic and early pregnant gilts; 2) the effect of estradiol (E-2) and progesterone (P-4) on HOXA10 expression in porcine luminal epithelial (LE) and stromal (ST) cells in vitro; 3) the effect of E2 and conceptus-exposed medium (CEM) on HOXA10 and prostaglandin endoperoxide synthase (PTGS2) gene expression and prostaglandin (PG) E-2 secretion from LE and ST cells in a co-culture model. The abundance of HOXA10 mRNA was increased on day 15 of pregnancy in comparison to day 15 of the estrous cycle. Moreover, increased HOXA10 mRNA level was detected in ST cells after E-2 and P-4 treatment. E-2 stimulated the expression of HOXA10 in LE cells cultured on collagen and pre-treated with steroids, but not in LE on plastic surfaces. Addition of CEM to LE cells cultured in collagen-coated inserts of the co-culture system resulted in elevated HOXA10 and PTGS2 gene expression and PGE(2) secretion in these cells, but not in ST cells cultured in basal compartments. ST cells directly treated with E-2 or CEM showed higher levels of HOXA10 and PTGS2 expression. Blocking of estrogen receptors with ICI-182,780 did not influence the stimulatory effect of CEM. We conclude that HOXA10 expression in the porcine endometrium is closely related to the implantation process and stimulated by conceptus products. Moreover, the co-culture system of LE and ST cells is a promising model for the study of endometrial response to conceptus-derived factors. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:954 / 966
页数:13
相关论文
共 52 条
[1]   Endometrial receptivity markers, the journey to successful embryo implantation [J].
Achache, Hanna ;
Revel, Ariel .
HUMAN REPRODUCTION UPDATE, 2006, 12 (06) :731-746
[2]   A HOXA10 estrogen response element (ERE) is differentially regulated by 17 beta-estradiol and diethylstilbestrol (DES) [J].
Akbas, GE ;
Song, J ;
Taylor, HS .
JOURNAL OF MOLECULAR BIOLOGY, 2004, 340 (05) :1013-1023
[3]   Expression of porcine endometrial prostaglandin synthase during the estrous cycle and early pregnancy, and following endocrine disruption of pregnancy [J].
Ashworth, Morgan D. ;
Ross, Jason W. ;
Hu, Jianbo ;
White, Frankie J. ;
Stein, Daniel R. ;
DeSilva, Udaya ;
Johnson, Gregory A. ;
Spencer, Thomas E. ;
Geisert, Rodney D. .
BIOLOGY OF REPRODUCTION, 2006, 74 (06) :1007-1015
[4]   Development of mouse embryos co-cultured with polarized or non-polarized uterine epithelial cells using sequential culture media [J].
Azadbakht, Mehri ;
Valojerdi, Mojtaba Rezazadeh ;
Mowla, Sayed Javad .
ANIMAL REPRODUCTION SCIENCE, 2007, 100 (1-2) :141-157
[5]  
Benson GV, 1996, DEVELOPMENT, V122, P2687
[6]   Role of tumour necrosis factor α in stimulation of prostaglandins F2α and E2 release by cultured porcine porcine endometrial cells [J].
Blitek, A. ;
Ziecik, A. J. .
REPRODUCTION IN DOMESTIC ANIMALS, 2006, 41 (06) :562-567
[7]   Expression of cyclooxygenase-1 and-2 in the porcine endometrium during the oestrous cycle and early pregnancy [J].
Blitek, A. ;
Waclawik, A. ;
Kaczmarek, M. M. ;
Stadejek, T. ;
Pejsak, Z. ;
Ziecik, A. J. .
REPRODUCTION IN DOMESTIC ANIMALS, 2006, 41 (03) :251-257
[8]   Effect of LH on prostaglandin F2α and prostaglandin E2 secretion by cultured porcine endometrial cells [J].
Blitek, A ;
Ziecik, AJ .
REPRODUCTION, 2005, 130 (01) :105-112
[9]   Prostaglandins F2α and E2 secretion by porcine epithelial and stromal endometrial cells on different days of the oestrous cycle [J].
Blitek, A ;
Ziecik, AJ .
REPRODUCTION IN DOMESTIC ANIMALS, 2004, 39 (05) :340-346
[10]   Effect of estrus induction on prostaglandin content and prostaglandin synthesis enzyme expression in the uterus of early pregnant pigs [J].
Blitek, A. ;
Waclawik, A. ;
Kaczmarek, M. M. ;
Kiewisz, J. ;
Ziecik, A. J. .
THERIOGENOLOGY, 2010, 73 (09) :1244-1256