Efficient acetone-butanol-ethanol production (ABE) by Clostridium acetobutylicum XY16 immobilized on chemically modified sugarcane bagasse

被引：21
作者：
Kong, Xiangping [1 ]
He, Aiyong [1 ]
Zhao, Jie [1 ]
Wu, Hao [1 ]
Jiang, Min [1 ]
机构：
[1] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Jiangsu, Peoples R China
来源：
BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2015年 / 38卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Modification; Sugarcane bagasse; Butanol; Clostridium acetobutylicum XY16; Polyethylenimine; FED-BATCH; FERMENTATION; ADSORPTION; BUTYRATE; BIOFUELS; REACTOR; CELLS; FIBER;
D O I：
10.1007/s00449-015-1377-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Sugarcane bagasse was chemically modified by polyethylenimine (PEI) and glutaraldehyde (GA) and then used as a support to immobilize Clostridium acetobutylicum XY16 in the process of butanol production. Compared with batch fermentation using unmodified sugarcane bagasse, 22.3 g/L total solvents were produced by cells immobilized on 4 g/L PEI treated sugarcane bagasse with high solvent productivity of 0.62 g/(L h) and glucose consumption rate of 1.67 g/(L h). Improvement of 14, 43, and 37 % in total solvent titer, solvent productivity and glucose consumption rate was observed, respectively. Enhanced solvent production of 25.14 g/L was obtained when using a high concentration of glucose of 80 g/L. Continuous fermentation was studied using PEI/GA modified sugarcane bagasse as immobilization support with a range of dilution which rates from 0.2 to 2.5 to find an optimal condition. The maximum solvent productivity of 11.32 g/(L h) was obtained at a high dilution rate of 2.0 h(-1).
引用
收藏
页码：1365 / 1372
页数：8
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