Purification, crystallization and preliminary X-ray structure analysis of the laccase from Ganoderma lucidum

被引:8
作者
Lyashenko, Andrey V. [1 ]
Belova, Oksana [2 ]
Gabdulkhakov, Azatg. [1 ]
Lashkov, Alexander A. [1 ]
Lisov, Alexandr V. [2 ]
Leontievsky, Alexey A. [2 ]
Mikhailov, Al'bert M. [1 ]
机构
[1] Russian Acad Sci, AV Shubnikov Inst Crystallog, Moscow 119333, Russia
[2] Russian Acad Sci, GK Skryabin Inst Biochem Physiol Microorganisms, Pushchino 142290, Russia
来源
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2011年 / 67卷
关键词
FUNGAL LACCASES; ANGSTROM RESOLUTION; CRYSTAL-STRUCTURE; MULTICOPPER OXIDASES; DIFFRACTION ANALYSIS; MOLECULAR-CLONING; TRAMETES-HIRSUTA; FULL COMPLEMENT; CERRENA-MAXIMA; BLUE LACCASE;
D O I
10.1107/S1744309111021129
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The ligninolytic enzymes of the basidiomycetes play a key role in the global carbon cycle. A characteristic property of these enzymes is their broad substrate specificity, which has led to their use in various biotechnologies, thus stimulating research into the three-dimensional structures of ligninolytic enzymes. This paper presents the purification, crystallization and preliminary X-ray analysis of the laccase from the ligninolytic basidiomycete Ganoderma lucidum.
引用
收藏
页码:926 / 929
页数:4
相关论文
共 42 条
[1]   Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta [J].
Abadulla, E ;
Tzanov, T ;
Costa, S ;
Robra, KH ;
Cavaco-Paulo, A ;
Gübitz, GM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (08) :3357-3362
[2]   Circular dichroism of prophenol oxidase in relation to the structural stability in Drosophila melanogaster [J].
Asada, N ;
Namba, M ;
Kodama, T ;
Kyogoku, Y .
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY, 2004, 56 (01) :1-6
[3]   Fungal laccases - occurrence and properties [J].
Baldrian, P .
FEMS MICROBIOLOGY REVIEWS, 2006, 30 (02) :215-242
[4]   Crystal structure of a four-copper laccase complexed with an arylamine: Insights into substrate recognition and correlation with kinetics [J].
Bertrand, T ;
Jolivalt, C ;
Briozzo, P ;
Caminade, E ;
Joly, N ;
Madzak, C ;
Mougin, C .
BIOCHEMISTRY, 2002, 41 (23) :7325-7333
[5]   OXIDATION OF NONPHENOLIC SUBSTRATES - AN EXPANDED ROLE FOR LACCASE IN LIGNIN BIODEGRADATION [J].
BOURBONNAIS, R ;
PAICE, MG .
FEBS LETTERS, 1990, 267 (01) :99-102
[6]   Efficient bleaching of non-wood high-quality paper pulp using laccase-mediator system [J].
Camarero, S ;
García, O ;
Vidal, T ;
Colom, J ;
del Río, JC ;
Gutiérrez, A ;
Gras, JM ;
Monje, R ;
Martínez, MJ ;
Martínez, AT .
ENZYME AND MICROBIAL TECHNOLOGY, 2004, 35 (2-3) :113-120
[7]   p-hydroxycinnamic acids as natural mediators for laccase oxidation of recalcitrant compounds [J].
Camarero, Susana ;
Canas, Ana I. ;
Nousiainen, Paula ;
Record, Eric ;
Lomascolo, Anne ;
Jesus Martinez, Maria ;
Martinez, Angel T. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2008, 42 (17) :6703-6709
[8]   Laccases and their occurrence in prokaryotes [J].
Claus, H .
ARCHIVES OF MICROBIOLOGY, 2003, 179 (03) :145-150
[9]  
DeLano W., 2020, PYMOL
[10]   Structure of the laccase from Coprinus cinereus at 1.68 Å resolution:: evidence for different 'type 2 Cu-depleted′ isoforms [J].
Ducros, V ;
Brzozowski, AM ;
Wilson, KS ;
Ostergaard, P ;
Schneider, P ;
Svendson, A ;
Davies, GJ .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 :333-336