A sensor based on gold nanoparticle/single-walled carbon nanotube film on the surface of glassy carbon electrode is prepared. Electrochemical behavior of adrenaline hydrochloride (AH) on the surface of gold nanoparticle/single-walled carbon nanotube modified glassy carbon electrode is investigated. A simple, sensitive, and inexpensive method for determination of AH is proposed. The oxidation peak currents is proportional to adrenaline hydrochloride concentrations in the range of 0.20 mg L-1 to 1.80 mg L-1 in 0.1 M phosphate buffer solution of pH 7.3, the detection limit for AH is 0.06 mg L-1, and the recoveries are in the range from 100.0 to 110.0% with RSD of 1.2-1.9% (n =6). (C) 2011 Elsevier B.V. All rights reserved.