Isolation, expression and immunological characterization of a calcium-binding protein from Parietaria pollen

被引：14

作者：

Bonura, A. ^{[1
]}

Gulino, L. ^{[1
]}

Trapani, A. ^{[1
]}

Di Felice, G. ^{[2
]}

Tinghino, R. ^{[2
]}

Amoroso, S. ^{[5
]}

Geraci, D. ^{[1
]}

Valenta, R. ^{[3
]}

Westritschnig, K. ^{[3
]}

Scala, E. ^{[4
]}

Mari, A. ^{[4
]}

Colombo, P. ^{[1
]}

机构：

[1] CNR, Ist Biomed & Immunol Mol Alberto Monroy, Palermo, Italy

[2] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, I-00161 Rome, Italy

[3] Med Univ Vienna, Christian Doppler Lab Allergy Res, Div Immunopathol, Dept Pathophysiol, Vienna, Austria

[4] IDI IRCCS, Ctr Clin & Expt Allergol, Rome, Italy

[5] Unita Osped Allergol, Osped Civ, Palermo, Italy

来源：

MOLECULAR IMMUNOLOGY | 2008年 / 45卷 / 09期

基金：

奥地利科学基金会;

关键词：

cross-reactivity; EF-hand; Parietaria pollen; polcalcin; recombinant allergen;

D O I：

10.1016/j.molimm.2008.01.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The diagnosis and therapy of allergic disorders are usually performed with crude extracts which are a heterogeneous mixture of proteins with different allergenic potency. The knowledge of the allergenic composition is a key step for diagnostic and therapeutic options. Parietaria judaica pollen represents one of the main sources of allergens in the Mediterranean area and its major allergens have already been identified (Par j 1 and Par j 2). In addition, inhibition studies performed using a calcium-binding protein (CBP) from grass pollen (Ph1 p 7) showed the presence of a homologue of this cross-reactive allergen in the Parietaria extract. Screening of a cDNA library allowed us to isolate a 480 bp cDNA containing the information for an 87 AA long protein with high level of homology to calcium-binding proteins from other allergenic sources. It was expressed as a recombinant allergen in Escherichia coli and purified by affinity chromatography. Its expression allowed us to study the prevalence of this allergen in a population of allergic patients in southern Europe. Immunoblotting and inhibition studies showed that this allergen shares a pattern of IgE epitopes in common with other 2-EF-hand calcium-binding proteins from botanically non-related species. The immunological properties of the Pj CBP were investigated by CD63 activation assay and CFDA-SE staining. In conclusion, DNA recombinant technology allowed the isolation, expression and immunological characterization of a cross-reactive calcium-binding protein allergen from Parietaria judaica pollen. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2465 / 2473

页数：9

共 34 条

[1]

Asturias JA, 2004, J INVEST ALLERG CLIN, V14, P43

[2] QUANTITATION OF THE MAJOR ALLERGEN OF SEVERAL PARIETARIA POLLENS BY AN ANTI-PAR-1 MONOCLONAL ANTIBODY-BASED ELISA - ANALYSIS OF CROSS-REACTIVITY AMONG PURIFIED PAR-J-1, PAR-O-1 AND PAR-M-1 ALLERGENS [J].

AYUSO, R ;

CARREIRA, J ;

POLO, F .

CLINICAL AND EXPERIMENTAL ALLERGY, 1995, 25 (10) :993-999

[3] Ole e 3, an olive-tree allergen, belongs to a widespread family of pollen proteins [J].

Batanero, E ;

Villalba, M ;

Ledesma, A ;

Puente, XS ;

Rodriguez, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 241 (03) :772-778

[4] Cross-reactivity between Parietaria species using the major rParj1 and rParj2 allergens [J].

Bonura, Angela ;

Artale, Anna ;

Marino, Mauro ;

Amoroso, Saverio ;

Marcucci, Francesco ;

Geraci, Domenico ;

Colombo, Paolo .

ALLERGY AND ASTHMA PROCEEDINGS, 2006, 27 (05) :378-382

[5] The allergens of Parietaria [J].

Colombo, P ;

Bonura, A ;

Costa, MA ;

Izzo, V ;

Passantino, R ;

Locorotondo, G ;

Amoroso, S ;

Geraci, D .

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2003, 130 (03) :173-179

[6]

Colombo P, 1998, J IMMUNOL, V160, P2780

[7] CDNA CLONING, EXPRESSION AND PRIMARY STRUCTURE OF PAR-J-I, A MAJOR ALLERGEN OF PARIETARIA-JUDAICA POLLEN [J].

COSTA, MA ;

COLOMBO, P ;

IZZO, V ;

KENNEDY, H ;

VENTURELLA, S ;

COCCHIARA, R ;

MISTRELLO, G ;

FALAGIANI, P ;

GERACI, D .

FEBS LETTERS, 1994, 341 (2-3) :182-186

[8] Allergenic pollen and pollen allergy in Europe [J].

D'Amato, G. ;

Cecchi, L. ;

Bonini, S. ;

Nunes, C. ;

Annesi-Maesano, I. ;

Behrendt, H. ;

Liccardi, G. ;

Popov, T. ;

van Cauwenberge, P. .

ALLERGY, 2007, 62 (09) :976-990

[9]

D'Amato G, 2000, CLIN EXP ALLERGY, V30, P628

[10] cDNA cloning, sequence analysis and allergological characterization of Par j 2.0101, a new major allergen of the Parietaria judaica pollen [J].

Duro, G ;

Colombo, P ;

Costa, MA ;

Izzo, V ;

Porcasi, R ;

DiFiore, R ;

Locorotondo, G ;

Mirisola, MG ;

Cocchiara, R ;

Geraci, D .

FEBS LETTERS, 1996, 399 (03) :295-298

← 1 2 3 4 →