High Throughput Screening and Selection Methods for Directed Enzyme Evolution

被引：150

作者：

Xiao, Han ^{[1
]}

Bao, Zehua ^{[2
]}

Zhao, Huimin ^{[1
,2
,3
,4
]}

机构：

[1] Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[4] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA

来源：

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH | 2015年 / 54卷 / 16期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

IN-VITRO SELECTION; RESONANCE ENERGY-TRANSFER; CELL-SURFACE DISPLAY; RIBOSOME DISPLAY; PHAGE DISPLAY; PROTEIN; SYSTEM; IDENTIFICATION; BIOCATALYSTS; SPECIFICITY;

D O I：

10.1021/ie503060a

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Successful evolutionary enzyme engineering requires a high throughput screening or selection method, which considerably increases the chance of obtaining desired properties and reduces the time and cost. In this review, a series of high throughput screening and selection methods are illustrated With significant and recent examples. These high throughput strategies are also discussed with an emphasis on compatibility with phenotypic analysis during directed enzyme evolution. Lastly, certain limitations of current methods, as well as future developments, are briefly summarized.

引用

页码：4011 / 4020

页数：10

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