Discrimination of neutral oligosaccharides through a nanopore

被引：30
作者：
Bacri, Laurent [1 ]
Oukhaled, Abdelghani [1 ]
Hemon, Erven [1 ]
Bassafoula, Fenseth Banzouzi [1 ]
Auvray, Loic [1 ]
Daniel, Regis [1 ]
机构：
[1] Univ Evry Val dEssonne, Lab Anal & Environm, CNRS UMR 8587, F-91025 Evry, France
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 412卷 / 04期
关键词：
Nanopore; alpha-Hemolysin; Oligosaccharides; Single molecule; Translocation; SINGLE-MOLECULE; HEPARAN-SULFATE; CONFORMATION;
D O I：
10.1016/j.bbrc.2011.07.121
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The detection of oligosaccharides at the single-molecule level was investigated using a protein nanopore device. Neutral oligosaccharides of various molecular weights were translocated through a single alpha-hemolysin nanopore and their nano-transit recorded at the single-molecule level. The translocation of maltose and dextran oligosaccharides featured by 1 -> 4 and 1 -> 6 glycosidic bonds respectively was studied in an attempt to discriminate oligosaccharides according to their polymerization degree and glycosidic linkages. Oligosaccharides were translocated through a free diffusion regime indicating that they adopted an extended conformation during their translocation in the nanopore. The dwell time increased with molecular mass, suggesting the usefulness of nanopore as a molecular sizing device. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：561 / 564
页数：4
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