Mechanical properties of individual focal adhesions probed with a magnetic microneedle

被引:98
作者
Matthews, BD
Overby, DR
Alenghat, FJ
Karavitis, J
Numaguchi, Y
Allen, PG
Ingber, DE [1 ]
机构
[1] Harvard Univ, Sch Med, Childrens Hosp, Vasc Biol Program,Dept Pathol, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Childrens Hosp, Dept Surg, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Pediat, Boston, MA 02115 USA
[4] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med,Div Hematol, Boston, MA 02115 USA
关键词
mechanical stress; integrins; focal adhesion; mechanotransduction; cell mechanics; magnetometry;
D O I
10.1016/j.bbrc.2003.12.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A permanent magnetic microneedle was developed to apply tensional forces to integrin receptors via ligand-coated magnetic microbeads while optically analyzing the mechanical properties of individual focal adhesions. Force application (130 pN for 3 s) through activated beta1 integrins produced less bead displacement than when unligated integrins were stressed. This strengthening response differed markedly on a bead-by-bead basis, correlated directly with local focal adhesion assembly, and was similar when analyzed at 4 degreesC, indicating that it was due to passive material properties of the cell. Viscoelastic analysis clarified that recruitment of focal adhesion proteins increased the local elastic stiffness of the adhesion complex without changing its viscous behavior. These data indicate that individual focal adhesions exhibit distinct mechanical properties that depend upon local focal adhesion assembly, and that these local variations in micromechanics can be detected and analyzed within living cells using the permanent magnetic microneedle technique. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:758 / 764
页数:7
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