Authentication and characterization of a candidate antagonistic bacterium against soft rot of Amorphophallus konjac

被引:21
作者
Wu, Jinping [1 ,2 ]
Liu, Xiaoyan [2 ]
Diao, Ying [1 ]
Ding, Zili [2 ]
Hu, Zhongli [1 ]
机构
[1] Wuhan Univ, Coll Life Sci, Minist Educ Plant Dev Biol, Key Lab, Wuhan 430072, Peoples R China
[2] Hubei Acad Agr Sci, Wuhan 430064, Peoples R China
关键词
Amorphophallus konjac; Biological control; Bacterial soft rot; Serratia marcescens; SERRATIA;
D O I
10.1016/j.cropro.2011.12.008
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Bacterial soft rot of Amorphophallus konjac is the most destructive disease caused by Pectobacterium spp. Until now effective control of this disease is unavailable. In the present study, attempts were made to find antagonistic bacteria to control the disease. After screening bacteria from infected konjac plants, a strain (21-2) was found to be able to effectively inhibit the pathogens of soft rot and it was identified as Serratia marcescens using a polyphasic approach including morphological characterization and analysis of the 16S rDNA gene sequence. In an HPLC MS analysis, the active substances of strain 21-2 were purified and separated from fermentation broth and divided into 36 constituents. The substances with a retention time of 17.35 min with the ultraviolet absorption peak wavelength at 211 nm and 261 nm and the molecular mass was 454 Da had the highest activity to inhibit the pathogens of soft rot on konjac. This constituent could be the potential biocontrol agent against the pathogens of soft rot on konjac. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:83 / 87
页数:5
相关论文
共 21 条
  • [1] Transgenic Amorphophallus konjac expressing synthesized acyl-homoserine lactonase (aiiA) gene exhibit enhanced resistance to soft rot disease
    Ban, Huifang
    Chai, Xinli
    Lin, Yongjun
    Zhou, Ying
    Peng, Donghai
    Zhou, Yi
    Zou, Yulan
    Yu, Ziniu
    Sun, Ming
    [J]. PLANT CELL REPORTS, 2009, 28 (12) : 1847 - 1855
  • [2] Chen X.W., 2005, TIANJIN AGR SCI, V11, P5
  • [3] David D.V., 2009, BMC PLANT BIOL, V9, P9, DOI [10.1186/1471-2229-9-9, DOI 10.1186/1471-2229-9-9]
  • [4] Complete Genome Sequence of Bacillus subtilis BSn5, an Endophytic Bacterium of Amorphophallus konjac with Antimicrobial Activity for the Plant Pathogen Erwinia carotovora subsp carotovora
    Deng, Yun
    Zhu, Yiguang
    Wang, Pengxia
    Zhu, Lei
    Zheng, Jinshui
    Li, Rong
    Ruan, Lifang
    Peng, Donghai
    Sun, Ming
    [J]. JOURNAL OF BACTERIOLOGY, 2011, 193 (08) : 2070 - 2071
  • [5] Fang Z.D., 1998, PLANT DIS RES
  • [6] Commercialization and implementation of biocontrol
    Fravel, DR
    [J]. ANNUAL REVIEW OF PHYTOPATHOLOGY, 2005, 43 : 337 - 359
  • [7] Garrity G.M., 2001, Bergey's Manual of Systematic Bacteriology
  • [8] Guo JianHua Guo JianHua, 2001, Chinese Journal of Biological Control, V17, P101
  • [9] Kalbe C, 1996, MICROBIOL RES, V151, P433, DOI 10.1016/S0944-5013(96)80014-0
  • [10] ECOLOGY OF THE SOFT ROT ERWINIAS
    PEROMBELON, MCM
    KELMAN, A
    [J]. ANNUAL REVIEW OF PHYTOPATHOLOGY, 1980, 18 : 361 - 387