Cross-talk between the Smad1 and Ras/MEK signaling pathways for TGFβ

Our previous data demonstrated that Ras activation was necessary and sufficient for transforming growth factor-p (TGF beta)-mediated Erk1 activation, and,vas required for TGF beta up-regulation of the Cdk inhibitors (CKI's) p27(Kip1) and p21(Cip1) (KM Mulder and SL Morris, J, Biol, Chem., 267, 5029-5031, 1992; MT Hartsough and KM Mulder, J, Biol, Chem., 270, 7117-7124, 1995; MT Hartsough ef nl,, J, Biol, Chem., 271, 22368-22375, 1996 and J Yue et al., Oncogene, 17, 47-55, 1998), Here we examined the role of Res in TGF beta-mediated effects on a rat homolog of Smad1 (termed RSmad1), We demonstrate that both TGF beta and bone morphogenetic protein (BMP) can induce endogenous Smad1 phosphorylation in intestinal epithelial cells (IECs), The combination of transient expression of RSmad1 and TGF beta treatment had an additive effect on induction of the TGF beta-responsive reporter 3TP-lux, Either inactivation of Ras by stable, inducible expression of a dominant-negative mutant of Ras (RasN17) or addition of MAP and ERK kinase (MEK) inhibitor PD98059 to cells significantly decreased the ability of both TGF beta and BMP to induce phosphorylation of endogenous Smad1 in IECs, Moreover, either inactivation of Ras or addition of PD98059 to IEC 4-1 cells inhibited the ability of RSmad1 to regulate 3TP luciferase activity in both the presence and absence of TGF beta, Collectively, our data indicate that TGF beta can regulate RSmad1 function in epithelial cells, and that the Ras/MEK pathway is partially required for TGF beta-mediated regulation of RSmad1.