Direct MALDI-TOF Mass Spectrometry Assay of Blood Culture Broths for Rapid Identification of Candida Species Causing Bloodstream Infections: an Observational Study in Two Large Microbiology Laboratories

被引：160

作者：

Spanu, Teresa ^{[1
]}

Posteraro, Brunella ^{[1
]}

Fiori, Barbara ^{[1
]}

D'Inzeo, Tiziana ^{[1
]}

Campoli, Serena ^{[1
]}

Ruggeri, Alberto ^{[1
]}

Tumbarello, Mario ^{[2
]}

Canu, Giulia ^{[1
]}

Trecarichi, Enrico Maria ^{[2
]}

Parisi, Gabriella ^{[3
]}

Tronci, Mirella ^{[3
]}

Sanguinetti, Maurizio ^{[1
]}

Fadda, Giovanni ^{[1
]}

机构：

[1] Univ Cattolica Sacro Cuore, Ist Microbiol, Rome, Italy

[2] Univ Cattolica Sacro Cuore, Ist Clin Malattie Infett, Rome, Italy

[3] Azienda Osped San Camillo Forlanini, Microbiol Lab, Rome, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 01期

关键词：

DESORPTION IONIZATION-TIME; INVASIVE CANDIDIASIS; YEAST; MICROORGANISMS; PREDICTORS; MORTALITY; BACTERIA; THERAPY; BOTTLES;

D O I：

10.1128/JCM.05742-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the reliability of the Bruker Daltonik's MALDI Biotyper system in species-level identification of yeasts directly from blood culture bottles. Identification results were concordant with those of the conventional culture-based method for 95.9% of Candida albicans (187/195) and 86.5% of non-albicans Candida species (128/148). Results were available in 30 min (median), suggesting that this approach is a reliable, time-saving tool for routine identification of Candida species causing bloodstream infection.

引用

页码：176 / 179

页数：4

共 18 条

[1] Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections
Pulcrano, Giovanna
Iula, Dora Vita
Vollaro, Antonio
Tucci, Alessandra
Cerullo, Monica
Esposito, Matilde
Rossano, Fabio
Catania, Maria Rosaria
JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 94 (03) : 262 - 266
[2] Direct identification of microorganisms in positive blood culture bottles by mass spectrometry (MALDI-TOF MS)
Azula, Natalia
Relloso, Silvia
Barrios, Ruben
Nicola, Federico
Smayevsky, Jorgelina
ACTA BIOQUIMICA CLINICA LATINOAMERICANA, 2022, 56 (01): : 17 - 31
[3] Rapid Identification and Susceptibility Testing of Candida spp. from Positive Blood Cultures by Combination of Direct MALDI-TOF Mass Spectrometry and Direct Inoculation of Vitek 2
Idelevich, Evgeny A.
Grunewald, Camilla M.
Wuellenweber, Joerg
Becker, Karsten
PLOS ONE, 2014, 9 (12):
[4] Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) directly from positive blood culture flasks allows rapid identification of bloodstream infections in immunosuppressed hosts
Egli, A.
Osthoff, M.
Goldenberger, D.
Halter, J.
Schaub, S.
Steiger, J.
Weisser, M.
Frei, R.
TRANSPLANT INFECTIOUS DISEASE, 2015, 17 (03) : 481 - 487
[5] Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
Gray, Timothy J.
Thomas, Lee
Olma, Tom
Mitchell, David H.
Iredell, Jon R.
Chen, Sharon C. A.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (87):
[6] Performance assessment of two lysis methods for direct identification of yeasts from clinical blood cultures using MALDI-TOF mass spectrometry
Jeddi, Fakhri
Yapo-Kouadio, Gisele Cha
Normand, Anne-Cecile
Cassagne, Carole
Marty, Pierre
Piarroux, Renaud
MEDICAL MYCOLOGY, 2017, 55 (02) : 185 - 192
[7] Work Flow Analysis of Around-the-clock Processing of Blood Culture Samples and Integrated MALDI-TOF Mass Spectrometry Analysis for the Diagnosis of Bloodstream Infections
Schneiderhan, Wilhelm
Grundt, Alexander
Woerner, Stefan
Findeisen, Peter
Neumaier, Michael
CLINICAL CHEMISTRY, 2013, 59 (11) : 1649 - 1656
[8] Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry
Gray, Timothy J.
Thomas, Lee
Olma, Tom
Iredell, Jonathan R.
Chen, Sharon C. -A.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2013, 77 (02) : 110 - 112
[9] Are We Ready for Nosocomial Candida auris Infections? Rapid Identification and Antifungal Resistance Detection Using MALDI-TOF Mass Spectrometry May Be the Answer
De Carolis, Elena
Marchionni, Federica
La Rosa, Marilisa
Meis, Jacques F.
Chowdhary, Anuradha
Posteraro, Brunella
Sanguinetti, Maurizio
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2021, 11
[10] Bacterial species identification using MALDI-TOF mass spectrometry and machine learning techniques: A large-scale benchmarking study
Mortier, Thomas
Wieme, Anneleen D.
Vandamme, Peter
Waegeman, Willem
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 2021, 19 : 6157 - 6168

← 1 2 →