Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells

被引：28

作者：

Jin, Meihua ^{[1
,2
]}

Zhao, Wennan ^{[1
,2
]}

Zhang, Yanwen ^{[1
,2
]}

Kobayashi, Motomasa ^{[3
]}

Duan, Hongquan ^{[1
,2
]}

Kong, Dexin ^{[1
,2
]}

机构：

[1] Tianjin Med Univ, Sch Pharmaceut Sci, Tianjin Key Lab Technol Enabling Dev Clin Therape, Tianjin 300070, Peoples R China

[2] Tianjin Med Univ, Res Ctr Basic Med Sci, Tianjin 300070, Peoples R China

[3] Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2011年 / 12卷 / 11期

关键词：

aaptamine; K562; cells; G2/M arrest; p21; SESQUITERPENE AMINOQUINONE; ERYTHROID-DIFFERENTIATION; MARINE SPONGE; BCR-ABL; SMENOSPONGINE; RESISTANCE; MECHANISMS; MUTATIONS;

D O I：

10.3390/ijms12117352

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 mu M, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells.

引用

页码：7352 / 7359

页数：8

共 16 条

[1] Aaptamine, a spongean alkaloid, activates p21 promoter in a p53-independent manner [J].