Immobilization of GOx Enzyme on SiO2-Coated Ni-Co Ferrite Nanocomposites as Magnetic Support and Their Antimicrobial and Photocatalytic Activities

The present study used a sol-gel auto-combustion approach to make silica (SiO2)-coated Ni-Co ferrite nanocomposites that would be used as a platform for enzyme immobilization. Using glutaraldehyde as a coupling agent, glucose oxidase (GOx) was covalently immobilized on this magnetic substrate. X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy (HRTEM), and fourier transform infrared spectroscopy (FTIR) was used to determine the structural analysis and morphology of Ni-Co ferrite/SiO2 nanocomposites. FTIR spectra confirmed the binding of GOx to Ni-Co ferrite/SiO2 nanocomposites, with a loading efficiency of around 85%. At alkaline pH and higher temperature, the immobilized GOx enzyme exhibited increased catalytic activity. After 10 times of reuses, it still had 69% catalytic activity. Overall, the immobilized GOx displayed higher operational stability than the free enzyme under severe circumstances and was easily recovered by magnetic separation. With increased doping concentration of the nanocomposites, the photocatalytic activity was assessed using a degradation process in the presence of methylene blue dye under UV light irradiation, which revealed that the surface area of the nanocomposites with increased doping concentration played a significant role in improving photocatalytic activity. The antibacterial activity of Ni-Co ferrite/SiO2 nanocomposites was assessed using the agar well diffusion method against Escherichia coli, a gram-negative bacteria (ATCC 25922). Consequently, it was revealed that doping of Ni2+ and Co2+ in Fe2O4 /SiO2 nanocomposites at varied concentrations improved their antibacterial properties.