Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response

被引:5
|
作者
Li, Teng [1 ,2 ,3 ,4 ]
Liu, Hua [1 ,2 ,3 ]
Jiang, Nan [1 ,2 ,3 ]
Wang, Yiluo [1 ,2 ,3 ]
Wang, Ying [1 ,2 ,3 ]
Zhang, Jing [1 ,2 ,3 ]
Shen, Yujuan [1 ,2 ,3 ,4 ]
Cao, Jianping [1 ,2 ,3 ,4 ]
机构
[1] Chinese Ctr Dis Control & Prevent, Chinese Trop Dis Res, Natl Inst Parasit Dis, Shanghai, Peoples R China
[2] Natl Hlth Commiss, Key Lab Parasite & Vector Biol, Shanghai, Peoples R China
[3] WHO, Collaborating Ctr Trop Dis, Shanghai, Peoples R China
[4] Shanghai Jiao Tong Univ, Sch Med, Sch Global Hlth, Chinese Ctr Trop Dis Res, Shanghai, Peoples R China
来源
PLOS NEGLECTED TROPICAL DISEASES | 2021年 / 15卷 / 11期
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
INFECTION; ICAM-1; BIOSYNTHESIS; APICOMPLEXAN; ENDOTHELIUM; ACTIVATION; INHIBITION; MORBIDITY; MORTALITY; DEFENSE;
D O I
10.1371/journal.pntd.0009949
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Cryptosporidium is a life-threating protozoan parasite belonging to the phylum Apicomplexa, which mainly causes gastroenteritis in a variety of vertebrate hosts. Currently, there is a reemergence of Cryptosporidium infection; however, no fully effective drug or vaccine is available to treat Cryptosporidiosis. In the present study, to better understand the detailed interaction between the host and Cryptosporidium parvum, a large-scale label-free proteomics study was conducted to characterize the changes to the proteome induced by C. parvum infection. Among 4406 proteins identified, 121 proteins were identified as differentially abundant (> 1.5-fold cutoff, P < 0.05) in C. parvum infected HCT-8 cells compared with uninfected cells. Among them, 67 proteins were upregulated, and 54 proteins were downregulated at 36 h post infection. Analysis of the differentially abundant proteins revealed an interferon-centered immune response of the host cells against C. parvum infection and extensive inhibition of metabolism-related enzymes in the host cells caused by infection. Several proteins were further verified using quantitative real-time reverse transcription polymerase chain reaction and western blotting. This systematic analysis of the proteomics of C. parvum-infected HCT-8 cells identified a wide range of functional proteins that participate in host anti-parasite immunity or act as potential targets during infection, providing new insights into the molecular mechanism of C. parvum infection.
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页数:19
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