Hybridization-Induced "Off-On" 19F-NMR Signal Probe Release from DNA-Functionalized Gold Nanoparticles

被引：21
作者：
Kieger, Alexander
Wiester, Michael J. [1 ,2 ]
Procissi, Daniel [3 ]
Parrish, Todd B. [3 ]
Mirkin, Chad A. [1 ,2 ]
Thaxton, C. Shad [4 ,5 ]
机构：
[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
[2] Northwestern Univ, Int Inst Nanotechnol, Evanston, IL 60208 USA
[3] Northwestern Univ, Feinberg Sch Med, Dept Radiol, Chicago, IL 60611 USA
[4] Northwestern Univ, Feinberg Sch Med, Dept Urol, Int Inst Nanotechnol, Chicago, IL 60611 USA
[5] Northwestern Univ, Feinberg Sch Med, Inst BioNanotechnol & Med, Chicago, IL 60611 USA
来源：
SMALL | 2011年 / 7卷 / 14期
关键词：
NANO-FLARES; METALLIC NANOPARTICLES; RNA DETECTION; TRANSFECTION;
D O I：
10.1002/smll.201100566
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Fluorine-19-DNA-functionalized gold nanoparticles ( 19F-DNA AuNPs) are demonstrated to provide a 19F-NMR off-on switch in response to target-specific DNA hybridization events. AuNP-bound 19F-DNA produces a low intensity signal that is undetectable above background. Upon complementary DNA hybridization and subsequent 19F-DNA release, the 19F-NMR signal becomes detectable. This method has the potential to be used both in vitro and in vivo to non-invasively detect and image specific nucleic acid binding events of interest. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1977 / 1981
页数：5
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