Analysis of Expression of Genes Involved in Immune Response Modulation in Silent Multipotent Mesenchymal Stromal Cells

被引：0
作者：
Petinati, N. A. [1 ]
Shipunova, I. N. [1 ]
Bigildeyev, A. E. [1 ]
Kuz'mina, L. A. [1 ]
Momotyuk, K. S. [1 ]
Parovichnikova, E. N. [1 ]
Drize, N. I. [1 ]
Savchenko, V. G. [1 ]
机构：
[1] Russian Acad Med Sci, Hematol Res Ctr, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2012年 / 153卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
multipotent mesenchymal stromal cells; polymerase chain reaction; gene expression; fibroblast colony forming units; STEM-CELLS; APLASTIC-ANEMIA; INTERLEUKIN-6; DISEASE;
D O I：
10.1007/s10517-012-1687-7
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The expression of some genes modulating the immune response was studied in multipotent mesenchymal stromal cells (MMSC) from the bone marrow of a healthy donor. Non-activated MMSC expressed IL-6 and IL-10, complement H factor, macrophage growth factor, prostaglandin E2 synthase, and indoleamine-2,3-dioxygenase. The expression of all these genes was higher in female MMSC. A close inverse relationship between IL-6 expression in MMSC and male donor age, close relationship between body weight index and fibroblast CFU concentration in female donor bone marrow and between indoleamine-2,3-dioxygenase and macrophage growth factor in MMSC from these donors were detected. The expression of the analyzed genes was higher in MMSC of donors who had no antibodies to cytomegalovirus, herpes simplex virus, and Epstein-Barr virus in the blood. The results demonstrate the MMSC regulation of immune reactions by MMSC at the cell and organism levels.
引用
收藏
页码：244 / 248
页数：5
相关论文
共 16 条
[1]   Mutations in BMP4 cause eye, brain, and digit developmental anomalies:: Overlap between the BMP4 and hedgehog signaling pathways [J].
Bakrania, Preeti ;
Efthymiou, Maria ;
Klein, Johannes C. ;
Salt, Alison ;
Bunyan, David J. ;
Wyatt, Alex ;
Ponting, Chris P. ;
Martin, Angela ;
Williams, Steven ;
Lindley, Victoria ;
Gilmore, Joanne ;
Restori, Marie ;
Robson, Anthony G. ;
Neveu, Magella M. ;
Holder, Graham E. ;
Collin, J. Richard O. ;
Robinson, David O. ;
Farndon, Peter ;
Johansen-Berg, Heidi ;
Gerrelli, Dianne ;
Ragge, Nicola K. .
AMERICAN JOURNAL OF HUMAN GENETICS, 2008, 82 (02) :304-319
[2]   MESENCHYMAL STEM-CELLS [J].
CAPLAN, AI .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1991, 9 (05) :641-650
[3]   Mesenchymal stem cells: building blocks for molecular medicine in the 21st century [J].
Caplan, AI ;
Bruder, SP .
TRENDS IN MOLECULAR MEDICINE, 2001, 7 (06) :259-264
[4]   Prostaglandin E2-mediated dysregulation of proinflammatory cytokine production in pristane-induced lupus mice [J].
Chae, Byeong Suk ;
Shin, Tae Yong ;
Kim, Dae Keun ;
Eun, Jae Soon ;
Leem, Jae Yoon ;
Yang, Jae Heon .
ARCHIVES OF PHARMACAL RESEARCH, 2008, 31 (04) :503-510
[5]   Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs) [J].
Cheng, HW ;
Jiang, W ;
Phillips, FM ;
Haydon, RC ;
Peng, Y ;
Zhou, L ;
Luu, HH ;
An, NL ;
Breyer, B ;
Vanichakarn, P ;
Szatkowski, JP ;
Park, JY ;
He, TC .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2003, 85A (08) :1544-1552
[6]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[7]   Immunosuppression by mesenchymal stromal cells: From culture to clinic [J].
Jones, Ben J. ;
McTaggart, Steven J. .
EXPERIMENTAL HEMATOLOGY, 2008, 36 (06) :733-741
[8]   Tissue engineering through autologous mesenchymal stem cells [J].
Jorgensen, C ;
Gordeladze, J ;
Noel, D .
CURRENT OPINION IN BIOTECHNOLOGY, 2004, 15 (05) :406-410
[9]   Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells [J].
Le Blanc, K ;
Rasmusson, I ;
Sundberg, B ;
Götherström, C ;
Hassan, M ;
Uzunel, M ;
Ringdén, O .
LANCET, 2004, 363 (9419) :1439-1441
[10]   Hnmunomodulatory properties of mesenchymal stromal cells [J].
Nauta, Alma J. ;
Fibbe, Willem E. .
BLOOD, 2007, 110 (10) :3499-3506
12