The virulence plasmid of Salmonella typhimurium is self-transmissible

被引：94

作者：

Ahmer, BMM ^{[1
]}

Tran, M ^{[1
]}

Heffron, F ^{[1
]}

机构：

[1] Oregon Hlth Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97201 USA

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 04期

关键词：

D O I：

10.1128/JB.181.4.1364-1368.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Most isolates of Salmonella enterica serovar Typhimurium contain a 90-kb virulence plasmid, This plasmid is reported to be mobilizable but nonconjugative, However, we have determined that the virulence plasmid of strains LT2, 14028, and SR-11 is indeed self-transmissible. The plasmid of strain SL1344 is not. Optimal conjugation frequency requires filter matings on M9 minimal glucose plates with a recipient strain lacking the virulence plasmid, These conditions result in a frequency of 2.9 x 10(-4) transconjugants/donor. Matings on Luria-Bertani plates, liquid matings, or matings with a recipient strain carrying the virulence plasmid reduce the efficiency by up to 400-fold. Homologs of the F plasmid conjugation genes are physically located on the virulence plasmid and are required for the conjugative phenotype.

引用

页码：1364 / 1368

页数：5

共 36 条

[11]

GUINEY DG, 1994, FEMS MICROBIOL LETT, V124, P1, DOI 10.1016/0378-1097(94)90324-7

[12] THE SALMONELLA-TYPHIMURIUM VIRULENCE PLASMID INCREASES THE GROWTH-RATE OF SALMONELLAE IN MICE [J].

GULIG, PA ;

DOYLE, TJ .

INFECTION AND IMMUNITY, 1993, 61 (02) :504-511

[13] MOLECULAR ANALYSIS OF SPV VIRULENCE GENES OF THE SALMONELLA VIRULENCE PLASMIDS [J].

GULIG, PA ;

DANBARA, H ;

GUINEY, DG ;

LAX, AJ ;

NOREL, F ;

RHEN, M .

MOLECULAR MICROBIOLOGY, 1993, 7 (06) :825-830

[14] MECHANISM OF RESISTANCE TO COMPLEMENT-MEDIATED KILLING OF BACTERIA ENCODED BY THE SALMONELLA-TYPHIMURIUM VIRULENCE PLASMID GENE RCK [J].

HEFFERNAN, EJ ;

REED, S ;

HACKETT, J ;

FIERER, J ;

ROUDIER, C ;

GUINEY, D .

JOURNAL OF CLINICAL INVESTIGATION, 1992, 90 (03) :953-964

[15] EPIDEMIOLOGY OF VIRULENCE-ASSOCIATED PLASMIDS AND OUTER-MEMBRANE PROTEIN-PATTERNS WITHIN 7 COMMON SALMONELLA SEROTYPES [J].

HELMUTH, R ;

STEPHAN, R ;

BUNGE, C ;

HOOG, B ;

STEINBECK, A ;

BULLING, E .

INFECTION AND IMMUNITY, 1985, 48 (01) :175-182

[16] AROMATIC-DEPENDENT SALMONELLA-TYPHIMURIUM ARE NON-VIRULENT AND EFFECTIVE AS LIVE VACCINES [J].

HOISETH, SK ;

STOCKER, BAD .

NATURE, 1981, 291 (5812) :238-239

[17] A proteinaceous gene regulatory thermometer in Salmonella [J].

Hurme, R ;

Berndt, KD ;

Normark, SJ ;

Rhen, M .

CELL, 1997, 90 (01) :55-64

[18] Transcriptional activation of Salmonella typhimurium invasion genes by a member of the phosphorylated response-regulator superfamily [J].

Johnston, C ;

Pegues, DA ;

Hueck, CJ ;

Lee, CA ;

Miller, SI .

MOLECULAR MICROBIOLOGY, 1996, 22 (04) :715-727

[19] ASSOCIATION OF ADHESIVE, INVASIVE, AND VIRULENT PHENOTYPES OF SALMONELLA-TYPHIMURIUM WITH AUTONOMOUS 60-MEGADALTON PLASMIDS [J].

JONES, GW ;

RABERT, DK ;

SVINARICH, DM ;

WHITFIELD, HJ .

INFECTION AND IMMUNITY, 1982, 38 (02) :476-486

[20] A PHYSICAL MAP OF THE SALMONELLA-TYPHIMURIUM LT2 GENOME MADE BY USING XBAI ANALYSIS [J].

LIU, SL ;

SANDERSON, KE .

JOURNAL OF BACTERIOLOGY, 1992, 174 (05) :1662-1672

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