The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient

被引:12
作者
Silva, Luciana Diniz [1 ]
Camargos Rocha, Andreia Maria [1 ]
Rocha, Gifone Aguiar [1 ]
de Moura, Silvia Beleza [4 ]
Negreiros Pinto Rocha, Marcia Maria [1 ]
Dani, Renato [5 ]
de Melo, Fabricio Freire [1 ,4 ]
Guerra, Juliana Becattini [1 ]
Fonseca de Castro, Lucia Porto [2 ]
Mendes, Guilherme Santiago [5 ]
de Abreu Ferrari, Teresa Cristina [3 ]
Lima, Agnaldo Soares [3 ]
Magalhaes Queiroz, Dulciene Maria [1 ]
机构
[1] Univ Fed Minas Gerais, Lab Pesquisa Bacteriol, Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Dept Anat Patol, Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Fac Med, Hosp Clin, Serv Transplante, Belo Horizonte, MG, Brazil
[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Belo Horizonte, MG, Brazil
[5] Hosp Governador Israel Pinheiro, Inst Previdencia Servidores Estado Minas Gerais, Belo Horizonte, MG, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2011年 / 106卷 / 06期
关键词
Helicobacter pylori; liver diseases; IL-10; IL-17; IFN-gamma; HEPATOCELLULAR-CARCINOMA; CHRONIC HEPATITIS; DISEASE; INFECTION; ASSOCIATION; CIRRHOSIS;
D O I
10.1590/S0074-02762011000600016
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The hypothesis that Helicobacter might be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacter in the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th) 1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-gamma and decreased IL-17A concentrations (p < 10(-3)). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.
引用
收藏
页码:748 / 754
页数:7
相关论文
共 23 条
[1]   Helicobacter pylori persistence:: An overview of interactions between H-pylori and host immune defenses [J].
Algood, Holly M. Scott ;
Cover, Timothy L. .
CLINICAL MICROBIOLOGY REVIEWS, 2006, 19 (04) :597-+
[2]  
Avenaud P, 2000, CANCER, V89, P1431, DOI 10.1002/1097-0142(20001001)89:7<1431::AID-CNCR4>3.0.CO
[3]  
2-5
[4]   Emerging role of IL-23/IL-17 axis in H pylori-associated pathology [J].
Caruso, Roberta ;
Pallone, Francesco ;
Monteleone, Giovanni .
WORLD JOURNAL OF GASTROENTEROLOGY, 2007, 13 (42) :5547-5551
[5]  
DElios MM, 1997, J IMMUNOL, V158, P962
[6]   Helicobacter infection in patients with HCV-related chronic hepatitis, cirrhosis, and hepatocellular carcinoma [J].
Dore, MP ;
Realdi, G ;
Mura, D ;
Graham, DY ;
Sepulveda, AR .
DIGESTIVE DISEASES AND SCIENCES, 2002, 47 (07) :1638-1643
[7]   Cytokines and chronic liver disease [J].
Fainboim, Leonardo ;
Chernavsky, Alejandra ;
Paladino, Natalia ;
Flores, Ana C. ;
Arruvito, Lourdes .
CYTOKINE & GROWTH FACTOR REVIEWS, 2007, 18 (1-2) :143-157
[8]   Helicobacter species ribosomal DNA recovered from the liver tissue of Chinese patients with primary hepatocellular carcinoma [J].
Fan, XG ;
Peng, XN ;
Huang, Y ;
Yakoob, J ;
Wang, ZM ;
Chen, YP .
CLINICAL INFECTIOUS DISEASES, 2002, 35 (12) :1555-1557
[9]  
LAFDIL F, 2010, CELL MOL IMMUNOL, V22, P1
[10]   The Interleukin-17 Pathway Is Involved in Human Alcoholic Liver Disease [J].
Lemmers, Arnaud ;
Moreno, Christophe ;
Gustot, Thierry ;
Marechal, Raphael ;
Degre, Delphine ;
Demetter, Pieter ;
de Nadai, Patricia ;
Geerts, Albert ;
Quertinmont, Eric ;
Vercruysse, Vincent ;
Le Moine, Olivier ;
Deviere, Jacques .
HEPATOLOGY, 2009, 49 (02) :646-657