The IsdA gene is necessary for sexual development inhibition by a salt in Aspergillus nidulans

被引:0
作者
Lee, DW
Kim, S
Kim, SJ
Han, DM
Jahng, KY
Chae, KS [3 ]
机构
[1] Chonbuk Natl Univ, Div Biol Sci, Chonju 561756, Chonbuk, South Korea
[2] Wonkwang Univ, Fac Life Sci, Iksan 570749, Chonbuk, South Korea
[3] Chonbuk Natl Univ, Inst Mol Biol & Genet, Chonju 561756, Chonbuk, South Korea
关键词
Aspergillus nidulans; lsdA; potassium chloride; sexual development; veA;
D O I
暂无
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Using one of 17 expressed sequence tags (ESTs) previously identified as specific to the late sexual developmental (LSD) stage of Aspergillus ninulans, a gene for the subject EST was isolated. The determined DNA sequences revealed an open reading frame encoding a 356 amino acid polypeptide which does not share a sequence similarity to previously identified genes or proteins. The isolated gene was named lsdA (late sexual development). since it was expressed abundantly at the LSD stage. The lsdA gene expression began earlier than at the LSD stage. Disruption of the lsdA gene in the veA(+) background strongly induced sexual development under conditions where sexual development in wild-type strains is almost completely inhibited. In contrast, in the veA1 background, an lsdA null mutant failed to show any phenotypic difference in sexual development under the various conditions tested. These results indicate that the lsdA gene may be responsible for inhibiting the sexual development of veA(+) strains by a high concentration of a salt.
引用
收藏
页码:237 / 243
页数:7
相关论文
共 25 条
[1]   Asexual sporulation in Aspergillus nidulans [J].
Adams, TH ;
Wieser, JK ;
Yu, JH .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (01) :35-+
[2]   ASCOCARPS OF ASPERGILLUS AND PENICILLIUM [J].
BENJAMIN, CR .
MYCOLOGIA, 1955, 47 (05) :669-687
[3]  
BOMFORD R, 1980, CLIN EXP IMMUNOL, V39, P426
[4]   A cyclin-dependent kinase family member (PHOA) is required to link developmental fate to environmental conditions in Aspergillus nidulans [J].
Bussink, HJ ;
Osmani, SA .
EMBO JOURNAL, 1998, 17 (14) :3990-4003
[5]  
Champe SP, 1981, FUNGAL SPORE MORPHOG, P255
[6]   Sexual development of Aspergillus nidulans in tryptophan auxotrophic strains [J].
Eckert, SE ;
Hoffmann, B ;
Wanke, C ;
Braus, GH .
ARCHIVES OF MICROBIOLOGY, 1999, 172 (03) :157-166
[7]   HULLE CELL DEVELOPMENT IN EMERICELLA-NIDULANS [J].
ELLIS, TT ;
REYNOLDS, DR ;
ALEXOPOULOS, CJ .
MYCOLOGIA, 1973, 65 (05) :1028-1035
[8]  
HAN D M, 1990, Korean Journal of Mycology, V18, P225
[9]  
Han Yoo Jeong, 1993, Korean Journal of Genetics, V15, P1
[10]   The rpl16a gene for ribosomal protein L16A identified from expressed sequence tags is differentially expressed during sexual development of Aspergillus nidulans [J].
Jeong, HY ;
Han, DM ;
Jahng, KY ;
Chae, KS .
FUNGAL GENETICS AND BIOLOGY, 2000, 31 (02) :69-78