Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics

被引：25

作者：

Hoedt, Esthelle ^{[1
,2
]}

Zhang, Guoan ^{[3
]}

Neubert, Thomas A. ^{[1
,2
]}

机构：

[1] NYU, Sch Med, Skirball Inst, Kimmel Ctr Biol & Med, New York, NY 10016 USA

[2] NYU, Sch Med, Dept Cell Biol, New York, NY 10016 USA

[3] Weill Cornell Med, Prote & Metabol Core Facil, New York, NY USA

来源：

ADVANCEMENTS OF MASS SPECTROMETRY IN BIOMEDICAL RESEARCH, 2ND EDITION | 2019年 / 1140卷

关键词：

Stable isotope labeling by amino acids in cell culture (SILAC); Metabolic labeling; Quantitative proteomics; Mass spectrometry; TYROSINE PHOSPHORYLATION; MASS-SPECTROMETRY; IN-VIVO; SIGNALING PATHWAY; SUPER-SILAC; PROTEIN QUANTITATION; INTERACTING PROTEINS; GLOBAL ANALYSIS; WIDE ANALYSIS; QUANTIFICATION;

D O I：

10.1007/978-3-030-15950-4_31

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful approach for high-throughput quantitative proteomics. SILAC allows highly accurate protein quantitation through metabolic encoding of whole cell proteomes using stable isotope labeled amino acids. Since its introduction in 2002, SILAC has become increasingly popular. In this chapter we review the methodology and application of SILAC, with an emphasis on three research areas: dynamics of posttranslational modifications, protein-protein interactions, and protein turnover.

引用

页码：531 / 539

页数：9

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