Diversity of an uncommon elastic hypersaline microbial mat along a small-scale transect

被引：7

作者：

Espinosa-Asuar, Laura ^{[1
]}

Monroy-Guzman, Camila ^{[1
]}

Madrigal-Trejo, David ^{[1
]}

Navarro-Miranda, Marisol ^{[1
]}

Sanchez-Perez, Jazmin ^{[1
]}

Buenrostro Munoz, Jhoselinne ^{[1
]}

Villar, Juan ^{[2
]}

Cifuentes Camargo, Julian Felipe ^{[2
]}

Kalambokidis, Maria ^{[1
]}

Esquivel-Hernandez, Diego A. ^{[1
]}

Viladomat Jasso, Mariette ^{[1
]}

Escalante, Ana E. ^{[3
]}

Velez, Patricia ^{[4
]}

Figueroa, Mario ^{[5
]}

Martinez-Cardenas, Anahi ^{[5
]}

Ramirez-Barahona, Santiago ^{[4
]}

Gasca-Pineda, Jaime ^{[1
]}

Eguiarte, Luis E. ^{[1
]}

Souza, Valeria ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Ecol, Dept Ecol Evolut, Cdmx, Mexico

[2] Pontificia Univ Javeriana, Bogota, Colombia

[3] Univ Nacl Autonoma Mexico, Inst Ecol, Lab Nacl Ciencias Sostenibilidad, Cdmx, Mexico

[4] Univ Nacl Autonoma Mexico, Inst Biol, Dept Bot, Cdmx, Mexico

[5] Univ Nacl Autonoma Mexico, Fac Quim, Cdmx, Mexico

来源：

PEERJ | 2022年 / 10卷

关键词：

Cuatro Cienegas Coahuila; Microbial communities; Phylogenetic diversity; Rare biosphere; Metabolome analysis; CUATRO CIENEGAS BASIN; COMPARATIVE METAGENOMICS; MOLECULAR ECOLOGY; SP-NOV; ANTIBIOTICS; COMMUNITIES; CONSERVATION; ENVIRONMENT; METABOLITES; TOLERANCE;

D O I：

10.7717/peerj.13579

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We evaluated the microbial diversity and metabolome profile of an uncommon hypersaline elastic microbial mat from Cuatro Cienegas Basin (CCB) in the Chihuahuan Desert of Coahuila, Mexico. We collected ten samples on a small scale transect (1.5-m) and described its microbial diversity through NGS-based ITS and 16S rDNA gene sequencing. A very low number of taxa comprised a considerable proportion of the mat and were shared across all sampling points, whereas the rare biosphere was more phylogenetically diverse (Faith's Phylogenetic Diversity (FPD) index) and phylogenetically disperse (using a null model distribution of Phylogenetic Species Clustering (nmdPSC)) than the abundant (high read count) taxa for both analyzed libraries. We also found a distinctive metabolome profile for each sample and were able to tentatively annotate several classes of compounds with relevant biological properties.

引用

页数：30

共 118 条

[1] The genome of Bacillus coahuilensis reveals adaptations essential for survival in the relic of an ancient marine environment [J].

Alcaraz, Luis David ;

Olmedo, Gabriela ;

Bonilla, German ;

Cerritos, Rene ;

Hernandez, Gustavo ;

Cruz, Alfredo ;

Ramirez, Enrique ;

Putonti, Catherine ;

Jimenez, Beatriz ;

Martinez, Eva ;

Lopez, Varinia ;

Arvizu, Jacqueline L. ;

Ayala, Francisco ;

Razo, Francisco ;

Caballero, Juan ;

Siefert, Janet ;

Eguiarte, Luis ;

Vielle, Jean-Philippe ;

Martinez, Octavio ;

Souza, Valeria ;

Herrera-Estrella, Alfredo ;

Herrera-Estrella, Luis .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (15) :5803-5808

[2] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[3]

[Anonymous], **DATA OBJECT**

[4] Reproducible molecular networking of untargeted mass spectrometry data using GNPS [J].

Aron, Allegra T. ;

Gentry, Emily C. ;

McPhail, Kerry L. ;

Nothias, Louis-Felix ;

Nothias-Esposito, Melissa ;

Bouslimani, Amina ;

Petras, Daniel ;

Gauglitz, Julia M. ;

Sikora, Nicole ;

Vargas, Fernando ;

van Der Hooft, Justin J. J. ;

Ernst, Madeleine ;

Bin Kang, Kyo ;

Aceves, Christine M. ;

Caraballo-Rodriguez, Andres Mauricio ;

Koester, Irina ;

Weldon, Kelly C. ;

Bertrand, Samuel ;

Roullier, Catherine ;

Sun, Kunyang ;

Tehan, Richard M. ;

Boya P, Cristopher A. ;

Christian, Martin H. ;

Gutierrez, Marcelino ;

Ulloa, Aldo Moreno ;

Mora, Javier Andres Tejeda ;

Mojica-Flores, Randy ;

Lakey-Beitia, Johant ;

Vasquez-Chaves, Victor ;

Zhang, Yilue ;

Calderon, Angela, I ;

Tayler, Nicole ;

Keyzers, Robert A. ;

Tugizimana, Fidele ;

Ndlovu, Nombuso ;

Aksenov, Alexander A. ;

Jarmusch, Alan K. ;

Schmid, Robin ;

Truman, Andrew W. ;

Bandeira, Nuno ;

Wang, Mingxun ;

Dorrestein, Pieter C. .

NATURE PROTOCOLS, 2020, 15 (06) :1954-1991

[5] Daryamides A-C, weakly cytotoxic polyketides from a marine-derived actinomycete of the genus Streptomyces strain CNQ-085 [J].

Asolkar, Ratnakar N. ;

Jensen, Paul R. ;

Kauffman, Christopher A. ;

Fenical, William .

JOURNAL OF NATURAL PRODUCTS, 2006, 69 (12) :1756-1759

[6]

Atta-ur-Rahmanro, 2005, BIOACT PROD L, V32

[7] Activation of microbial secondary metabolic pathways: Avenues and challenges [J].

Baral, Bikash ;

Akhgari, Amir ;

Metsa-Ketela, Mikko .

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2018, 3 (03) :163-178

[8] Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory: Simulating Earth's present and past field environments [J].

Bebout, BM ;

Carpenter, SP ;

Des Marais, DJ ;

Discipulo, M ;

Embaye, T ;

Garcia-Pichel, F ;

Hoehler, TM ;

Hogan, M ;

Jahnke, LL ;

Keller, RM ;

Miller, SR ;

Prufert-Bebout, LE ;

Raleigh, C ;

Rothrock, M ;

Turk, K .

ASTROBIOLOGY, 2002, 2 (04) :383-402

[9] Complete genome sequence and description of Salinispira pacifica gen. nov., sp nov., a novel spirochaete isolated form a hypersaline microbial mat [J].

Ben Hania, Wajdi ;

Joseph, Manon ;

Schumann, Peter ;

Bunk, Boyke ;

Fiebig, Anne ;

Sproeer, Cathrin ;

Klenk, Hans-Peter ;

Fardeau, Marie-Laure ;

Spring, Stefan .

STANDARDS IN GENOMIC SCIENCES, 2015, 10

[10] UBER DIE ISOLIERUNG VON VERRUCARIN H VERRUCARIN J RORIDIN D UND RORIDIN E AUS MYROTHECIUM-ARTEN - VERRUCARINE UND RORIDINE .8. [J].

BOHNER, B ;

FETZ, E ;

HARRI, E ;

SIGG, HP ;

STOLL, C ;

TAMM, C .

HELVETICA CHIMICA ACTA, 1965, 48 (05) :1079-&

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