Orthogonal nanoarchitectonics of M13 phage for receptor targeted anticancer photodynamic therapy

被引：32

作者：

Ulfo, Luca ^{[1
]}

Cantelli, Andrea ^{[2
]}

Petrosino, Annapaola ^{[1
]}

Costantini, Paolo Emidio ^{[1
]}

Nigro, Michela ^{[1
]}

Starinieri, Francesco ^{[1
]}

Turrini, Eleonora ^{[3
]}

Zadran, Suleman Khan ^{[1
]}

Zuccheri, Giampaolo ^{[1
]}

Saporetti, Roberto ^{[2
]}

Di Giosia, Matteo ^{[2
]}

Danielli, Alberto ^{[1
]}

Calvaresi, Matteo ^{[2
]}

机构：

[1] Alma Mater Studiorum Univ Bologna, Dipartimento Farm & Biotecnol, Via Francesco Selmi 3, I-40126 Bologna, Italy

[2] Alma Mater Studiorum Univ Bologna, Dipartimento Chim Giacomo Ciamician, Via Francesco Selmi 2, I-40126 Bologna, Italy

[3] Alma Mater StudiorumUniv Bologna, Dipartimento Sci Qualita Vita, Corso Augusto 237, I-47921 Rimini, Italy

来源：

NANOSCALE | 2022年 / 14卷 / 03期

关键词：

CANCER-CELLS; GOLD NANOPARTICLES; FILAMENTOUS PHAGE; BACTERIOPHAGE; PHOTOSENSITIZERS; THERAPEUTICS; RECOGNITION; PEPTIDES; TEMPLATE; DELIVERY;

D O I：

10.1039/d1nr06053h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Photodynamic therapy (PDT) represents a promising therapeutic modality for cancer. Here we used an orthogonal nanoarchitectonics approach (genetic/chemical) to engineer M13 bacteriophages as targeted vectors for efficient photodynamic killing of cancer cells. M13 was genetically refactored to display on the phage tip a peptide (SYPIPDT) able to bind the epidermal growth factor receptor (EGFR). The refactored M13(EGFR) phages demonstrated EGFR-targeted tropism and were internalized by A431 cancer cells, that overexpress EGFR. Using an orthogonal approach to the genetic display, M13(EGFR) phages were then chemically modified, conjugating hundreds of Rose Bengal (RB) photosensitizing molecules on the capsid surface, without affecting the selective recognition of the SYPIPDT peptides. Upon internalization, the M13(EGFR)-RB derivatives generated intracellularly reactive oxygen species, activated by an ultralow intensity white light irradiation. The killing activity of cancer cells is observed at picomolar concentrations of the M13(EGFR) phage.

引用

页码：632 / 641

页数：11

共 83 条

[1] Evolutionary Selection of New Breast Cancer Cell-Targeting Peptides and Phages with the Cell-Targeting Peptides Fully Displayed on the Major Coat and Their Effects on Actin Dynamics during Cell Internalization [J].