Ultrasensitive Biosensor for Detection of Mercury(II) Ions Based on DNA-Cu Nanoclusters and Exonuclease III-assisted Signal Amplification

被引：13
作者：
Zhang, Hao [1 ]
Guan, Yanan [1 ]
Li, Xiaoshuang [1 ]
Lian, Lili [1 ]
Wang, Xiyue [1 ]
Gao, Wenxiu [1 ]
Zhu, Bo [1 ]
Liu, Xuying [1 ]
Lou, Dawei [1 ]
机构：
[1] Jilin Inst Chem Technol, Dept Analyt Chem, 45 Chengde St, Jilin 132022, Jilin, Peoples R China
来源：
ANALYTICAL SCIENCES | 2018年 / 34卷 / 10期
基金：
中国国家自然科学基金;
关键词：
Mercury(II) ion detection; copper nanoclusters; signal amplification; fluorescent biosensor; SENSITIVE FLUORESCENCE DETECTION; TEMPLATED COPPER NANOPARTICLES; HG2+ DETECTION; QUANTUM DOTS; LIVING CELLS; ASSAY; POLYMERASE; HG(II); PROBE; ACID;
D O I：
10.2116/analsci.18P124
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This paper describes a novel method for label-free mercury(II) ion detection based on exonuclease III-induced target signal recycling amplification using double-stranded DNA templated copper nanoclusters. The synthesized DNA-Cu nanoclusters were used with exonuclease III loop amplification technology for ultra-high sensitivity detection of mercury(II) ions, which were detected by significantly decreased fluorescence intensity. Under the optimal experimental conditions, there was a clear linear relationship between Hg2+ concentration in the range of 0.04 to 8 nM and fluorescence intensity. The detection limit for Hg2+ was 4 pM. In addition, the interference of other metal ions on the mercury(II) ion detection was also studied. To confirm the application of the fluorescent sensor, it was applied to determine the concentrations of mercury(II) ions in tap water, and the results showed that the method can be used to detect mercury(II) ions in water samples successfully.
引用
收藏
页码：1155 / 1161
页数：7
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