Determination of hippuric acid in human urine by ion chromatography with conductivity detection

被引：20
|
作者：
Zhao, Fuyong [1 ,2 ]
Wang, Zonghua [2 ]
Wang, Hui [1 ]
Ding, Mingyu [1 ]
机构：
[1] Tsinghua Univ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ, Dept Chem, Beijing 100084, Peoples R China
[2] Qingdao Univ, Growing Base State Key Lab, Lab Fiber Mat & Modern Text, Qingdao 266071, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2011年 / 879卷 / 3-4期
关键词：
Ion chromatography; Hippuric acid; Urine; GAS-CHROMATOGRAPHY; EXPOSURE; MIXTURE; XYLENES; WORKERS; TOLUENE;
D O I：
10.1016/j.jchromb.2010.12.015
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple, rapid, precise and eco-friendly ion chromatography (IC) method for the determination of hippuric acid (HA) in human urine was proposed in this paper. The separation was carried out an anion exchange column with 2.0 mmol L-1 Na2CO3 + 2.0 mmol L-1 NaHCO3 as mobile phase at the flow-rate 0.7 mL min(-1). A suppressed conductivity detector was used and the detection limit was 1.0 mu g L-1(S/N = 3) for hippuric acid. The analysis time for one run was 30 min under the optimized IC condition. The recovery of hippuric acid was 93.2-98.0% while the relative standard deviation (RSD) was 1.4-2.3% by seven measurements. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：296 / 298
页数：3
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