Structural Basis of Biological N2O Generation by Bacterial Nitric Oxide Reductase

被引：270

作者：

Hino, Tomoya ^{[1
,2
,3
]}

Matsumoto, Yushi ^{[1
,4
]}

Nagano, Shingo ^{[1
,5
]}

Sugimoto, Hiroshi ^{[1
]}

Fukumori, Yoshihiro ^{[6
]}

Murata, Takeshi ^{[2
,3
]}

Iwata, So ^{[2
,3
,7
]}

Shiro, Yoshitsugu ^{[1
]}

机构：

[1] RIKEN SPring 8 Ctr, Sayo, Hyogo 6795148, Japan

[2] Japan Sci & Technol Agcy, Human Receptor Crystallog Project, Sakyo Ku, Kyoto 6068501, Japan

[3] Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Kyoto 6068501, Japan

[4] Kyushu Univ, Med Inst Bioregulat, Postgenome Sci Ctr, Div Prot Chem, Fukuoka 8128552, Japan

[5] Tottori Univ, Grad Sch Engn, Dept Chem & Biotechnol, Tottori 6808552, Japan

[6] Kanazawa Univ, Grad Sch Nat Sci & Technol, Kanazawa, Ishikawa 9201192, Japan

[7] Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, Membrane Prot Crystallog Grp, London SW7 2AZ, England

来源：

SCIENCE | 2010年 / 330卷 / 6011期

基金：

日本科学技术振兴机构;

关键词：

CYTOCHROME-C-OXIDASE; PARACOCCUS-DENITRIFICANS; SPECTROSCOPIC CHARACTERIZATION; PSEUDOMONAS-AERUGINOSA; NO REDUCTASE; BC COMPLEX; HEME; PATHWAY; GLUTAMATES; STUTZERI;

D O I：

10.1126/science.1195591

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nitric oxide reductase (NOR) is an iron-containing enzyme that catalyzes the reduction of nitric oxide (NO) to generate a major greenhouse gas, nitrous oxide (N2O). Here, we report the crystal structure of NOR from Pseudomonas aeruginosa at 2.7 angstrom resolution. The structure reveals details of the catalytic binuclear center. The non-heme iron (Fe-B) is coordinated by three His and one Glu ligands, but a His-Tyr covalent linkage common in cytochrome oxidases (COX) is absent. This structural characteristic is crucial for NOR reaction. Although the overall structure of NOR is closely related to COX, neither the D-nor K-proton pathway, which connect the COX active center to the intracellular space, was observed. Protons required for the NOR reaction are probably provided from the extracellular side.

引用

页码：1666 / 1670

页数：5

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