MiR-25 regulates cell proliferation and metastasis in bladder urothelial carcinoma

被引:2
作者
Ning, Jin-zhuo [1 ]
Chu, Chuan-min [2 ]
Du, Yang [1 ]
Zuo, Li [3 ]
机构
[1] Wuhan Univ, Dept Urol, Renmin Hosp, Wuhan, Hubei, Peoples R China
[2] Naval Med Univ, Dept Urol, Affiliated Hosp 3, Shanghai, Peoples R China
[3] Nanjing Med Univ, Affiliated Changzhou 2 Peoples Hosp, Dept Urol, Changzhou 213000, Jiangsu, Peoples R China
来源
JOURNAL OF CANCER | 2021年 / 12卷 / 22期
关键词
miR-25; PTEN; bladder cancer; tumorigenesis; prolifertation; metastasis;
D O I
10.7150/jca.62743
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Bladder urothelial carcinoma (BC) is a common malignant tumor with a high incidence. This study aims to explore the role of miR-25 in BC tumorigenesis. Material and Methods: The expression of miR-25 and PTEN were detected in clinical BC tissues. BC cell lines T24 and 5637 were used to transfect miR-25 mimics or inhibitors. Luciferase reporter gene detection confirmed the correlation between miR-25 and PTEN. CCK-8 method and flow cytometry were used to detect cell viability and apoptosis. Cell migration and invasion ability were examined by transwell assays. Western blotting detects the protein levels of PTEN, beta-catenin, GSK-3 beta and p-GSK-3 beta. Results: MiR-25 and PTEN expression are found to be negatively correlated in BC tissues. Further research confirmed that PTEN is a direct target of miR-25. In addition, the overexpression of miR-25 down-regulates the expression of PTEN, induces cell survival and inhibits apoptosis, while the knockout of miR-25 leads to the opposite result. miR-25 also inhibits the phosphorylation of GSK-3 beta and beta-catenin without changing the total level of GSK-3 beta. In vivo experiments confirmed that miR-25 plays an oncogene's role by regulating the PTEN and Wnt/beta-catenin signaling pathways. Conclusion: Our research shows that miR-25 has a negative regulatory effect on the expression of PTEN in clinical specimens and in vitro. miR-25 can promote the proliferation of BC cells and induce cell invasion. Therefore, miR-25 may be used as a biomarker to predict the progression of BC.
引用
收藏
页码:6706 / 6714
页数:9
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