Structures of Escherichia coli DNA mismatch repair enzyme MutS in complex with different mismatches:: a common recognition mode for diverse substrates

被引：123

作者：

Natrajan, G ^{[1
]}

Lamers, MH ^{[1
]}

Enzlin, JH ^{[1
]}

Winterwerp, HHK ^{[1
]}

Perrakis, A ^{[1
]}

Sixma, TK ^{[1
]}

机构：

[1] Netherlands Canc Inst, Div Mol Carcinogenesis, NL-1066 CX Amsterdam, Netherlands

来源：

NUCLEIC ACIDS RESEARCH | 2003年 / 31卷 / 16期

关键词：

D O I：

10.1093/nar/gkg677

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have refined a series of isomorphous crystal structures of the Escherichia coli DNA mismatch repair enzyme MutS in complex with G:T, A:A, C:A and G:G mismatches and also with a single unpaired thymidine. In all these structures, the DNA is kinked by similar to60degrees upon protein binding. Two residues widely conserved in the MutS family are involved in mismatch recognition. The phenylalanine, Phe 36, is seen stacking on one of the mismatched bases. The same base is also seen forming a hydrogen bond to the glutamate Glu 38. This hydrogen bond involves the N7 if the base stacking on Phe 36 is a purine and the N3 if it is a pyrimidine (thymine). Thus, MutS uses a common binding mode to recognize a wide range of mismatches.

引用

页码：4814 / 4821

页数：8

共 38 条

[21] Structure of bacteriorhodopsin at 1.55 Å resolution [J].

Luecke, H ;

Schobert, B ;

Richter, HT ;

Cartailler, JP ;

Lanyi, JK .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 291 (04) :899-911

[22]

Lynch HT, 1999, J MED GENET, V36, P801

[23] Raster3D: Photorealistic molecular graphics [J].

Merritt, EA ;

Bacon, DJ .

MACROMOLECULAR CRYSTALLOGRAPHY, PT B, 1997, 277 :505-524

[24] Mismatch repair in replication fidelity, genetic recombination, and cancer biology [J].

Modrich, P ;

Lahue, R .

ANNUAL REVIEW OF BIOCHEMISTRY, 1996, 65 :101-133

[25] Refinement of macromolecular structures by the maximum-likelihood method [J].

Murshudov, GN ;

Vagin, AA ;

Dodson, EJ .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1997, 53 :240-255

[26] Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA [J].

Obmolova, G ;

Ban, C ;

Hsieh, P ;

Yang, W .

NATURE, 2000, 407 (6805) :703-710

[27] Processing of X-ray diffraction data collected in oscillation mode [J].

Otwinowski, Z ;

Minor, W .

MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :307-326

[28] Nearest-neighbor thermodynamics and NMR of DNA sequences with internal A•A, C•C, G•G, and T•T mismatches [J].

Peyret, N ;

Seneviratne, PA ;

Allawi, HT ;

SantaLucia, J .

BIOCHEMISTRY, 1999, 38 (12) :3468-3477

[29] THE CRYSTAL-STRUCTURE OF HAEIII METHYLTRANSFERASE COVALENTLY COMPLEXED TO DNA - AN EXTRAHELICAL CYTOSINE AND REARRANGED BASE-PAIRING [J].

REINISCH, KM ;

CHEN, L ;

VERDINE, GL ;

LIPSCOMB, WN .

CELL, 1995, 82 (01) :143-153

[30] The Phe-X-Glu DNA binding motif of MutS -: The role of hydrogen bonding in mismatch recognition [J].

Schofield, MJ ;

Brownewell, FE ;

Nayak, S ;

Du, CW ;

Kool, ET ;

Hsieh, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (49) :45505-45508

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