A "Turn-on" fluorescent bioprobe with aggregation-induced emission characteristics for detection of influenza virus-specific hemagglutinin protein

被引：12

作者：

Pan, Xiaoling ^{[1
]}

Liu, Pai ^{[1
]}

Wu, Xinghui ^{[1
]}

Zhang, Yahui ^{[2
]}

Cai, Zhengxu ^{[1
]}

Shi, Jianbing ^{[1
]}

Zhi, Junge ^{[3
]}

Li, Zi ^{[4
]}

Wang, Dayan ^{[4
]}

Tong, Bin ^{[1
]}

Dong, Yuping ^{[1
]}

机构：

[1] Beijing Inst Technol, Beijing Key Lab Construct Tailorable Adv Funct Ma, Sch Mat Sci & Engn, 5 South Zhongguancun St, Beijing 100081, Peoples R China

[2] Beijing Inst Technol, Sch Life Sci, 5 South Zhongguancun St, Beijing 100081, Peoples R China

[3] Beijing Inst Technol, Sch Chem, 5 South Zhongguancun St, Beijing 100081, Peoples R China

[4] Natl Hlth & Family Planning Commiss, Natl Inst Viral Dis Control & Prevent, Collaborat Innovat Ctr Diag & Treatment Infect Di, Chinese Ctr Dis Control & Prevent, Beijing 102206, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2021年 / 345卷

关键词：

Hexaphenyl-1,3-butadiene derivatives; Aggregation-induced emission; Hemagglutinin; Turn-on" fluorescent response; REAL-TIME; QUANTUM DOTS; A VIRUS; PROBE; TRACKING; ASSAY; DISCOVERY; FEATURES; BINDING; PCR;

D O I：

10.1016/j.snb.2021.130392

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited "turn-on" fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.

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页数：8

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