Prevalence of 25-hydroxyvitamin D2 in Western New York: A 3-year study

Background: We evaluated the distribution of 25OH-D-2 and 25OH-D-3 in a general patient population in Western New York to provide insights into how common detectable vitamin D-2 is among samples from a general patient population. Methods: Serum 25OH-D-2 and 25OH-D-3 results measured by LC-MS/MS from June 2009 to December 2012 were retrospectively analyzed. Results: A total of 266,269 serum tests were included for analysis. The percentage of tests with 25OH-D-2 levels above the assay limit of quantitation (LoQ) decreased from 32% to 17% over the course of the study period. The percentage of tests with 25OH-D-2 levels higher than those of 25OH-D-3 decreased from 21% to 12%. Sixty-seven percent of the test results with 25OH-D-2 levels above the LoQ had serum concentrations of 25OH-D-2 higher than those of 25OH-D-3. Conclusion: Prevalence of tests with quantifiable 25OH-D-2 decreased over time and yet 17% of them still had detectable levels of 25OH-D-2, 67% of which had 25OH-D-2 levels higher than 25OH-D-3. To achieve accurate 25-hydroxyvitamin D measurement, clinical laboratories should assess the accuracy of their assays, and if necessary, determine the local prevalence of 25OH-D-2 to determine if mass spectrometry is the platform of choice to assess vitamin D deficiency. (C) 2015 Elsevier B.V. All rights reserved.