Upregulation of Plasmid Genes during Stationary Phase in Synechocystis sp Strain PCC 6803, a Cyanobacterium

被引：26

作者：

Berla, Bertram M. ^{[1
]}

Pakrasi, Himadri B. ^{[1
,2
]}

机构：

[1] Washington Univ, Dept Energy Environm & Chem Engn, St Louis, MO 63130 USA

[2] Washington Univ, Dept Biol, St Louis, MO 63130 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 15期

关键词：

ROLLING CIRCLE MECHANISM; ESCHERICHIA-COLI; PCC-6803; EXPRESSION; SYNECHOCOCCUS; RECOMBINANT; PROMOTERS; BACTERIA; SEQUENCE; VECTOR;

D O I：

10.1128/AEM.01174-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We analyzed DNA microarrays to identify highly expressed genes during stationary-phase growth of Synechocystis sp. PCC 6803. Many identified genes are on endogenous plasmids, with copy numbers between 0.4 and 7 per chromosome. The promoters of such genes will be useful for synthetic biology applications with this phototrophic host.

引用

页码：5448 / 5451

页数：4

共 25 条

[1] Production of phloroglucinol by Escherichia coli using a stationary-phase promoter [J].

Cao, Yujin ;

Xian, Mo .

BIOTECHNOLOGY LETTERS, 2011, 33 (09) :1853-1858

[2] Growth-phase dependent differential gene expression in Synechocystis sp strain PCC 6803 and regulation by a group 2 sigma factor [J].

Foster, Jamie S. ;

Singh, Abhay K. ;

Rothschild, Lynn J. ;

Sherman, Louis A. .

ARCHIVES OF MICROBIOLOGY, 2007, 187 (04) :265-279

[3] Genome-scale modeling of Synechocystis sp PCC 6803 and prediction of pathway insertion [J].

Fu, Pengcheng .

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2009, 84 (04) :473-483

[4] Effects of fatty acid activation on photosynthetic production of fatty acid-based biofuels in Synechocystis sp PCC6803 [J].

Gao, Qianqian ;

Wang, Weihua ;

Zhao, Hui ;

Lu, Xuefeng .

BIOTECHNOLOGY FOR BIOFUELS, 2012, 5

[5] Progress Toward a Biomimetic Leaf: 4,000 h of Hydrogen Production by Coating-Stabilized Nongrowing Photosynthetic Rhodopseudomonas palustris [J].

Gosse, Jimmy L. ;

Engel, Brian J. ;

Hui, Jeremy C. -H. ;

Harwood, Caroline S. ;

Flickinger, Michael C. .

BIOTECHNOLOGY PROGRESS, 2010, 26 (04) :907-918

[6] Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli [J].

Haddadin, FT ;

Harcum, SW .

BIOTECHNOLOGY AND BIOENGINEERING, 2005, 90 (02) :127-153

[7] Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology [J].

Huang, Hsin-Ho ;

Camsund, Daniel ;

Lindblad, Peter ;

Heidorn, Thorsten .

NUCLEIC ACIDS RESEARCH, 2010, 38 (08) :2577-2593

[8] Low-Copy Plasmids can Perform as Well as or Better Than High-Copy Plasmids for Metabolic Engineering of Bacteria [J].

Jones, Kristala L. ;

Kim, Seon-Won ;

Keasling, J. D. .

METABOLIC ENGINEERING, 2000, 2 (04) :328-338

[9] A putative sensor kinase, Hik31, is involved in the response of Synechocystis sp strain PCC 6803 to the presence of glucose [J].

Kahlon, S ;

Beeri, K ;

Ohkawa, H ;

Hihara, Y ;

Murik, O ;

Suzuki, I ;

Ogawa, T ;

Kaplan, A .

MICROBIOLOGY-SGM, 2006, 152 :647-655

[10] Structural analysis of four large Plasmids harboring in a unicellular cyanobacterium, Synechocystis sp PCC 6803 [J].

Kaneko, T ;

Nakamura, Y ;

Sasamoto, S ;

Watanabe, A ;

Kohara, M ;

Matsumoto, M ;

Shimpo, S ;

Yamada, M ;

Tabata, S .

DNA RESEARCH, 2003, 10 (05) :221-228

← 1 2 3 →