A corrected space group for Sulfolobus sulfataricus 5′-deoxy-5′-methylthioadenosine phosphorylase II

被引：2

作者：

Zhang, Yang ^{[1
]}

Zwart, Peter H. ^{[2
]}

Ealick, Steven E. ^{[1
]}

机构：

[1] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2012年 / 68卷

关键词：

PURINE NUCLEOSIDE PHOSPHORYLASE; 3-DIMENSIONAL STRUCTURE; CRYSTAL-STRUCTURE; DISULFIDE BONDS; SOLFATARICUS; REFINEMENT; RESOLUTION;

D O I：

10.1107/S0907444911051699

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

5'-Deoxy-5'-methylthioadenosine phosphorylase (MTAP) catalyzes the phosphorolytic cleavage of 5'-deoxy-5'-methylthioadenosine ( MTA), a byproduct of polyamine biosynthesis. The Sulfolobus sulfataricus genome encodes two MTAPs. SsMTAP I has broad substrate specifity, accepting guanosine, inosine, adenosine and MTA, while SsMTAP II is specific for MTA. SsMTAP I forms a donut-shaped hexamer, while SsMTAP II is a hexamer formed from trimers packed face to face. The structure of SsMTAP II was originally determined in space group P1 (PDB entry 2a8y) and showed R32 pseudosymmetry. Post-analysis using phenix. xtriage showed that the correct space group is C2. Here, the structure refined in space group C2 is reported and the factors that initially led to the incorrect space-group assignment are discussed.

引用

页码：249 / 252

页数：4

共 20 条

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