Characterization of Hippo Pathway Components by Gene Inactivation

被引：226
作者：
Plouffe, Steven W. [1 ,2 ]
Meng, Zhipeng [1 ,2 ]
Lin, Kimberly C. [1 ,2 ]
Lin, Brian [1 ,2 ]
Hong, Audrey W. [1 ,2 ]
Chun, Justin V. [1 ,2 ]
Guan, Kun-Liang [1 ,2 ]
机构：
[1] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Moores Canc Ctr, La Jolla, CA 92093 USA
来源：
MOLECULAR CELL | 2016年 / 64卷 / 05期
关键词：
TUMOR-SUPPRESSOR; CELL-PROLIFERATION; SIGNALING PATHWAY; YAP ONCOPROTEIN; GROWTH-CONTROL; TISSUE-GROWTH; SIZE-CONTROL; HUMAN CANCER; KINASES; DROSOPHILA;
D O I：
10.1016/j.molcel.2016.10.034
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Hippo pathway is important for regulating tissue homeostasis, and its dysregulation has been implicated in human cancer. However, it is not well under-stood how the Hippo pathway becomesdysregulated because few mutations in core Hippo pathway components have been identified. Therefore, much work in the Hippo field has focused on identifying upstream regulators, and a complex Hippo interactome has been identified. Nevertheless, it is not always clear which components are the most physiologically relevant in regulating YAP/TAZ. To provide an overview of important Hippo pathway components, we created knockout cell lines for many of these components and compared their relative contributions to YAP/TAZ regulation in response to a wide range of physiological signals. By this approach, we provide an overview of the functional importance of many Hippo pathway components and demonstrate NF2 and RHOA as important regulators of YAP/TAZ and TAOK1/3 as direct kinases for LATS1/2.
引用
收藏
页码：993 / 1008
页数：16
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