Silibinin inhibits the migration and invasion of human gastric cancer SGC7901 cells by downregulating MMP-2 and MMP-9 expression via the p38MAPK signaling pathway

被引：39

作者：

Lu, Shuming ^{[1
]}

Zhang, Zhuqing ^{[2
]}

Chen, Meiru ^{[3
]}

Li, Chunyan ^{[3
]}

Liu, Lina ^{[3
]}

Li, Yan ^{[1
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Gastroenterol, 36 Sanhao St, Shenyang 110004, Liaoning, Peoples R China

[2] Dalian Municipal Cent Hosp, Dept Pathol, Dalian 116033, Liaoning, Peoples R China

[3] Dalian Med Univ, Dept Gastroenterol, Affiliated Hosp 1, Dalian 116011, Liaoning, Peoples R China

来源：

ONCOLOGY LETTERS | 2017年 / 14卷 / 06期

关键词：

silibinin; gastric cancer; matrix metalloproteinase; p38MAPK; ACTIVATED PROTEIN-KINASE; FACTOR-KAPPA-B; MATRIX METALLOPROTEINASES; UP-REGULATION; MAP KINASE; P38; MAPK; SB203580; METASTASIS; APOPTOSIS; TUMORS;

D O I：

10.3892/ol.2017.7080

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The objective of the present study was to observe the effects of silibinin and the p38 mitogen-activated protein kinase (MAPK) signaling pathway inhibitor SB203580 on the migration and invasion capabilities of SGC7901 cells, and to explore the underlying associated mechanisms. Scratch, Transwell and Matrigel invasion assays were performed to study the effects of silibinin on cell migration and invasion. Western blot analysis was used to determine the expression levels of p38MAPK, phosphorylated (p-) p38MAPK, matrix metalloproteinase (MMP)-2 and MMP-9. At the genomic level, quantitative polymerase chain reaction was performed to evaluate the expression levels of MMP-2 and MMP-9. The results of scratch assay indicated that silibinin inhibited the migration capabilities of human gastric cancer SGC7901 cells in a dose-dependent manner. Additionally, Matrigel invasion and Transwell migration assays revealed that silibinin and SB203580 combined treatment significantly reduced the number of invasive cells. Western blot analysis indicated a reduced phosphorylation of p38MAPK without marked changes in p38MAPK expression. In addition, the expression of MMP-2 and MMP-9 significantly decreased in the presence of silibinin, SB203580, and the combination of silibinin and SB203580. In summary, silibinin decreased the invasion and migration abilities of SGC7901 cells by downregulating the expression of MMP-2 and MMP-9 through inhibiting p38MAPK signaling cascades.

引用

页码：7577 / 7582

页数：6

共 38 条

[1] Inhibition of the cardiac p38-MAPK pathway by SB203580 delays ischemic cell death [J].